Title :

Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines

Author(s) :

Drolez, Aurore [Auteur]
Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique [LBHE]
Vandenhaute, Elodie [Auteur]
Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique [LBHE]
Julien, Sylvain [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Institut National de la Santé et de la Recherche Médicale [INSERM]
Gosselet, Fabien [Auteur]
Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique [LBHE]
Burchell, Joy [Auteur]
King‘s College London
Cecchelli, Roméo [Auteur]
Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique [LBHE]
Delannoy, Philippe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Dehouck, Marie-Pierre [Auteur]
Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique [LBHE]
Mysiorek, Caroline [Auteur]
Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique [LBHE]
julien, sylvain [Auteur]

Journal title :

PLoS One

Volume number :

11

Pages :

e0151155

Publication date :

2016-03-09

ISSN :

1932-6203

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Around 7–17% of metastatic breast cancer patients will develop brain metastases, associated with a poor prognosis. To reach the brain parenchyma, cancer cells need to cross the highly restrictive endothelium of the Blood-Brain ...
Show more >Around 7–17% of metastatic breast cancer patients will develop brain metastases, associated with a poor prognosis. To reach the brain parenchyma, cancer cells need to cross the highly restrictive endothelium of the Blood-Brain Barrier (BBB). As treatments for brain metastases are mostly inefficient, preventing cancer cells to reach the brain could provide a relevant and important strategy. For that purpose an in vitro approach is required to identify cellular and molecular interaction mechanisms between breast cancer cells and BBB endothelium, notably at the early steps of the interaction. However, while numerous studies are performed with in vitro models, the heterogeneity and the quality of BBB models used is a limitation to the extrapolation of the obtained results to in vivo context, showing that the choice of a model that fulfills the biological BBB characteristics is essential. Therefore, we compared pre-established and currently used in vitro models from different origins (bovine, mice, human) in order to define the most appropriate tool to study interactions between breast cancer cells and the BBB. On each model, the BBB properties and the adhesion capacities of breast cancer cell lines were evaluated. As endothelial cells represent the physical restriction site of the BBB, all the models consisted of endothelial cells from animal or human origins. Among these models, only the in vitro BBB model derived from human stem cells both displayed BBB properties and allowed measurement of meaningful different interaction capacities of the cancer cell lines. Importantly, the measured adhesion and transmigration were found to be in accordance with the cancer cell lines molecular subtypes. In addition, at a molecular level, the inhibition of ganglioside biosynthesis highlights the potential role of glycosylation in breast cancer cells adhesion capacities.Show less >

Language :

Anglais

Audience :

Non spécifiée

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Régulation de la glycosylation terminale

Submission date :

2020-02-12T15:44:41Z
2021-03-05T10:30:31Z