Interaction study between HCV NS5A-D2 and NS5B using 19F NMR

Dujardin, Marie; Cantrelle, Francois-Xavier; Lippens, Guy; Hanoulle, Xavier

Document type :

Article dans une revue scientifique

DOI :

10.1007/s10858-017-0159-9

Permalink :

http://hdl.handle.net/20.500.12210/18581

Title :

Interaction study between HCV NS5A-D2 and NS5B using 19F NMR

Author(s) :

Dujardin, Marie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Cantrelle, Francois-Xavier [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Lippens, Guy [Auteur]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]
Hanoulle, Xavier [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Journal title :

Journal of Biomolecular NMR

Volume number :

Pages :

67-76

Publication date :

2018-01

ISSN :

0925-2738, 1573-5001

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

The non structural protein 5A (NS5A) regulates the replication of the hepatitis C viral RNA through a direct molecular interaction of its domain 2 (NS5A-D2) with the RNA dependent RNA polymerase NS5B. Because of conflicting ...
Show more >The non structural protein 5A (NS5A) regulates the replication of the hepatitis C viral RNA through a direct molecular interaction of its domain 2 (NS5A-D2) with the RNA dependent RNA polymerase NS5B. Because of conflicting data in the literature, we study here this molecular interaction using fluorinated versions of the NS5A-D2 protein derived from the JFH1 Hepatitis C Virus strain. Two methods to prepare fluorine-labelled NS5A-D2 involving the biosynthetic incorporation of a 19F-tryptophan using 5-fluoroindole and the posttranslational introduction of fluorine by chemical conjugation of 2-iodo-N-(trifluoromethyl)acetamide with the NS5A-D2 cysteine side chains are presented. The dissociation constants (KD) between NS5A-D2 and NS5B obtained with these two methods are in good agreement, and yield values comparable to those derived previously from a surface plasmon resonance study. We compare benefits and limitations of both labeling methods to study the interaction between an intrinsically disordered protein and a large molecular target by 19F NMR.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
Université de Lille

Collections :

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

RMN et interactions moléculaires

Submission date :

2020-02-12T15:44:48Z
2024-02-19T08:38:02Z
2024-02-19T08:43:38Z

Version	Link	Modification date
3	20.500.12210/18581*	2024-02-19T08:43:27Z
2	20.500.12210/18581.2	2024-02-19T08:34:42Z
1	20.500.12210/18581.1	2020-02-12T15:44:48Z

Interaction study between HCV NS5A-D2 and ...

BibTeX

CSV

Excel

RIS

Version history

Interaction study between HCV NS5A-D2 and ... BibTeX CSV Excel RIS

Version history

Interaction study between HCV NS5A-D2 and ...

BibTeX

CSV

Excel

RIS