Title :

Studying Intrinsically Disordered Proteins under True In Vivo Conditions by Combined Cross-Polarization and Carbonyl-Detection NMR Spectroscopy

Author(s) :

Lopez, Juan [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Pontificia Universidad Católica del Perú = Pontifical Catholic University of Peru [PUCP]
Schneider, Robert [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Cantrelle, Francois-Xavier [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Huvent, Isabelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Lippens, Guy [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés [LISBP]

Journal title :

Angewandte Chemie International Edition

Volume number :

55

Pages :

7418-7422

Publication date :

2016-06-20

ISSN :

1433-7851

English keyword(s) :

Alpha-synuclein
Carbon detection
Cross-polarization
In-cell NMR spectroscopy
Intrinsically disordered proteins

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

Under physiological conditions, studies of intrinsically disordered proteins (IDPs) by conventional NMR methods based on proton detection are severely limited by fast amide‐proton exchange with water. 13C detection has ...
Show more >Under physiological conditions, studies of intrinsically disordered proteins (IDPs) by conventional NMR methods based on proton detection are severely limited by fast amide‐proton exchange with water. 13C detection has been proposed as a solution to the exchange problem, but is hampered by low sensitivity. We propose a new pulse sequence combining proton–nitrogen cross‐polarization and carbonyl detection to record high‐resolution, high‐sensitivity NMR spectra of IDPs under physiological conditions. To demonstrate the efficacy of this approach, we recorded a high‐quality N–CO correlation spectrum of α‐synuclein in bacterial cells at 37 °C.Show less >

Language :

Anglais

Audience :

Non spécifiée

ANR Project :

Développement de stratégies innovantes pour une approche transdisciplinaire de la maladie d'Alzheime

Administrative institution(s) :

CNRS
Université de Lille

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

RMN et interactions moléculaires

Submission date :

2020-02-12T15:44:52Z
2021-04-23T08:45:55Z