In-cell NMR: from metabolites to macromolecules

Lippens, Guy; Cahoreau, E.; Millard, Pierre; Charlier, C.; Lopez, Juan; Hanoulle, Xavier; Portais, J. C.

Document type :

Article dans une revue scientifique

DOI :

10.1039/C7AN01635B

Permalink :

http://hdl.handle.net/20.500.12210/18620

Title :

In-cell NMR: from metabolites to macromolecules

Author(s) :

Lippens, Guy [Auteur]
Université de Toulouse [UT]
Cahoreau, E. [Auteur]
Université de Toulouse [UT]
Millard, Pierre [Auteur]
Université de Toulouse [UT]
Charlier, C. [Auteur]
Lopez, Juan [Auteur]
Pontificia Universidad Católica del Perú = Pontifical Catholic University of Peru [PUCP]
Hanoulle, Xavier [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Portais, J. C. [Auteur]
Université de Toulouse [UT]

Journal title :

The Analyst

Volume number :

143

Pages :

620-629

Publication date :

2018

ISSN :

0003-2654, 1364-5528

Keyword(s) :

Imagerie par résonance magnétique
Macromolécules

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

In-cell NMR of macromolecules has gained momentum over the last ten years as an approach that might bridge the branches of cell biology and structural biology. In this review, we put it in the context of earlier efforts ...
Show more >In-cell NMR of macromolecules has gained momentum over the last ten years as an approach that might bridge the branches of cell biology and structural biology. In this review, we put it in the context of earlier efforts that aimed to characterize by NMR the cellular environment of live cells and their intracellular metabolites. Although technical aspects distinguish these earlier in vivo NMR studies and the more recent in cell NMR efforts to characterize macromolecules in a cellular environment, we believe that both share major concerns ranging from sensitivity and line broadening to cell viability. Approaches to overcome the limitations in one subfield thereby can serve the other one and vice versa. The relevance in biomedical sciences might stretch from the direct following of drug metabolism in the cell to the observation of target binding, and thereby encompasses in-cell NMR both of metabolites and macromolecules. We underline the efforts of the field to move to novel biological insights by some selected examples.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CNRS
Université de Lille

Collections :

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

RMN et interactions moléculaires

Submission date :

2020-02-12T15:45:01Z
2021-05-21T13:09:08Z
2024-02-28T09:16:17Z

Version	Link	Modification date
3	20.500.12210/18620*	2024-02-28T09:11:12Z
2	20.500.12210/18620.2	2021-05-21T13:00:47Z
1	20.500.12210/18620.1	2020-02-12T15:45:01Z

In-cell NMR: from metabolites to macromolecules

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In-cell NMR: from metabolites to macromolecules

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