Microbial diversity observed during hemp retting
Document type :
Article dans une revue scientifique
Permalink :
Title :
Microbial diversity observed during hemp retting
Author(s) :
Ribeiro, Alexandra [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Pochart, Philippe [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Day, Arnaud [Auteur]
Mennuni, Sarah [Auteur]
Bono, Pierre [Auteur]
Baret, Jean-Luc [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Spadoni, Jean-Louis [Auteur]
Laboratoire génomique, bioinformatique et applications [GBA]
Mangin, Irène [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Pochart, Philippe [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Day, Arnaud [Auteur]

Mennuni, Sarah [Auteur]
Bono, Pierre [Auteur]
Baret, Jean-Luc [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Spadoni, Jean-Louis [Auteur]
Laboratoire génomique, bioinformatique et applications [GBA]
Mangin, Irène [Auteur]
Conservatoire National des Arts et Métiers [CNAM] [CNAM]
Journal title :
Applied Microbiology and Biotechnology
Volume number :
99
Pages :
4471-4484
Publication date :
2015-05
English keyword(s) :
Hemp retting
Microbiota
Molecular inventory
qPCR
Microbiota
Molecular inventory
qPCR
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. However, their applications are limited since ...
Show more >Historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. However, their applications are limited since manufacturers have some difficulties to standardise fabrication processes. This study is a first step before selection and isolation of strains that could later be used to optimise microbial retting efficiency and hence fibre quality. We studied six samples harvested on different ground types, at different dates and with different retting durations on field to obtain an exhaustive representation of the process. After DNA extraction, total bacteria and fungi associated with stems during retting were specifically quantified using real-time PCR. Then, using sequence analysis of randomly cloned 16S and 18S ribosomal RNA (rRNA) genes, a phylogenetic characterisation of the dominant microorganisms was carried out. Quantitatively, we showed that there were 8.1–9.5 log10 16S rRNA gene copies per gram of hemp straw for bacteria and 8.6–9.6 log10 18S rRNA gene copies per gram for fungi. Qualitatively, we noticed a higher bacterial diversity in comparison to fungi. This work showed that in the different samples, the same species were present but in significantly different proportions according to ground type, harvest dates and retting durations on field. The most frequent bacterial sequences were affiliated to species Escherichia coli, Pantoea agglomerans, Pseudomonas rhizosphaerae, Rhodobacter sp., Pseudomonas fulva, Rhizobium huautlense and Massilia timonae, whereas fungal sequences were principally related to the genera Cladosporium and Cryptococcus.Show less >
Show more >Historically used in textile and paper industry, hemp fibres have started to find new applications in composite materials with important economic and ecological advantages. However, their applications are limited since manufacturers have some difficulties to standardise fabrication processes. This study is a first step before selection and isolation of strains that could later be used to optimise microbial retting efficiency and hence fibre quality. We studied six samples harvested on different ground types, at different dates and with different retting durations on field to obtain an exhaustive representation of the process. After DNA extraction, total bacteria and fungi associated with stems during retting were specifically quantified using real-time PCR. Then, using sequence analysis of randomly cloned 16S and 18S ribosomal RNA (rRNA) genes, a phylogenetic characterisation of the dominant microorganisms was carried out. Quantitatively, we showed that there were 8.1–9.5 log10 16S rRNA gene copies per gram of hemp straw for bacteria and 8.6–9.6 log10 18S rRNA gene copies per gram for fungi. Qualitatively, we noticed a higher bacterial diversity in comparison to fungi. This work showed that in the different samples, the same species were present but in significantly different proportions according to ground type, harvest dates and retting durations on field. The most frequent bacterial sequences were affiliated to species Escherichia coli, Pantoea agglomerans, Pseudomonas rhizosphaerae, Rhodobacter sp., Pseudomonas fulva, Rhizobium huautlense and Massilia timonae, whereas fungal sequences were principally related to the genera Cladosporium and Cryptococcus.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Collections :
Research team(s) :
Fibres végétales
Submission date :
2020-02-12T15:45:18Z
2021-03-25T11:29:48Z
2021-03-25T11:31:41Z
2021-03-25T11:29:48Z
2021-03-25T11:31:41Z