A Cell Wall Proteome and Targeted Cell ...
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Article dans une revue scientifique
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Title :
A Cell Wall Proteome and Targeted Cell Wall Analyses Provide Novel Information on Hemicellulose Metabolism in Flax
Author(s) :
CHABI, MALIKA [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Goulas, Estelle [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Leclercq, Celine C. [Auteur]
Luxembourg Institute of Science and Technology [LIST]
De Waele, Isabelle [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Rihouey, Christophe [Auteur]
Université de Rouen Normandie [UNIROUEN]
Cenci, Ugo [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Day, Arnaud [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Blervacq, Anne-Sophie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Neutelings, Godfrey [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Duponchel, Ludovic [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Lerouge, Patrice [Auteur]
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
Université de Rouen Normandie [UNIROUEN]
Hausman, Jean-François [Auteur]
Luxembourg Institute of Science and Technology [LIST]
Renaut, Jenny [Auteur]
Luxembourg Institute of Science and Technology [LIST]
Hawkins, Simon [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Goulas, Estelle [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Leclercq, Celine C. [Auteur]
Luxembourg Institute of Science and Technology [LIST]
De Waele, Isabelle [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Rihouey, Christophe [Auteur]
Université de Rouen Normandie [UNIROUEN]
Cenci, Ugo [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Day, Arnaud [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Blervacq, Anne-Sophie [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Neutelings, Godfrey [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Duponchel, Ludovic [Auteur]

Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Lerouge, Patrice [Auteur]
Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale [Glyco-MEV]
Université de Rouen Normandie [UNIROUEN]
Hausman, Jean-François [Auteur]
Luxembourg Institute of Science and Technology [LIST]
Renaut, Jenny [Auteur]
Luxembourg Institute of Science and Technology [LIST]
Hawkins, Simon [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Journal title :
Molecular & Cellular Proteomics
Volume number :
16
Pages :
1634-1651
Publication date :
2017-07-13
ISSN :
1535-9476, 1535-9484
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Experimentally-generated (nanoLC-MS/MS) proteomic analyses of four different flax organs/tissues (inner-stem, outer-stem, leaves and roots) enriched in proteins from 3 different sub-compartments (soluble-, membrane-, and ...
Show more >Experimentally-generated (nanoLC-MS/MS) proteomic analyses of four different flax organs/tissues (inner-stem, outer-stem, leaves and roots) enriched in proteins from 3 different sub-compartments (soluble-, membrane-, and cell wall-proteins) was combined with publically available data on flax seed and whole-stem proteins to generate a flax protein database containing 2996 nonredundant total proteins. Subsequent multiple analyses (MapMan, CAZy, WallProtDB and expert curation) of this database were then used to identify a flax cell wall proteome consisting of 456 nonredundant proteins localized in the cell wall and/or associated with cell wall biosynthesis, remodeling and other cell wall related processes. Examination of the proteins present in different flax organs/tissues provided a detailed overview of cell wall metabolism and highlighted the importance of hemicellulose and pectin remodeling in stem tissues. Phylogenetic analyses of proteins in the cell wall proteome revealed an important paralogy in the class IIIA xyloglucan endo-transglycosylase/hydrolase (XTH) family associated with xyloglucan endo-hydrolase activity. Immunolocalisation, FT-IR microspectroscopy, and enzymatic fingerprinting indicated that flax fiber primary/S1 cell walls contained xyloglucans with typical substituted side chains as well as glucuronoxylans in much lower quantities. These results suggest a likely central role of xyloglucans and endotransglucosylase/hydrolase activity in flax fiber formation and cell wall remodeling processes.Show less >
Show more >Experimentally-generated (nanoLC-MS/MS) proteomic analyses of four different flax organs/tissues (inner-stem, outer-stem, leaves and roots) enriched in proteins from 3 different sub-compartments (soluble-, membrane-, and cell wall-proteins) was combined with publically available data on flax seed and whole-stem proteins to generate a flax protein database containing 2996 nonredundant total proteins. Subsequent multiple analyses (MapMan, CAZy, WallProtDB and expert curation) of this database were then used to identify a flax cell wall proteome consisting of 456 nonredundant proteins localized in the cell wall and/or associated with cell wall biosynthesis, remodeling and other cell wall related processes. Examination of the proteins present in different flax organs/tissues provided a detailed overview of cell wall metabolism and highlighted the importance of hemicellulose and pectin remodeling in stem tissues. Phylogenetic analyses of proteins in the cell wall proteome revealed an important paralogy in the class IIIA xyloglucan endo-transglycosylase/hydrolase (XTH) family associated with xyloglucan endo-hydrolase activity. Immunolocalisation, FT-IR microspectroscopy, and enzymatic fingerprinting indicated that flax fiber primary/S1 cell walls contained xyloglucans with typical substituted side chains as well as glucuronoxylans in much lower quantities. These results suggest a likely central role of xyloglucans and endotransglucosylase/hydrolase activity in flax fiber formation and cell wall remodeling processes.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
ENSCL
CNRS
Université de Lille
CNRS
Université de Lille
Collections :
Research team(s) :
Fibres végétales
Submission date :
2020-02-12T15:45:20Z
2021-05-28T09:42:24Z
2021-10-08T15:38:32Z
2021-05-28T09:42:24Z
2021-10-08T15:38:32Z
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