The spatio-temporal dynamics of PKA activity ...
Type de document :
Article dans une revue scientifique
URL permanente :
Titre :
The spatio-temporal dynamics of PKA activity profile during mitosis and its correlation to chromosome segregation
Auteur(s) :
Vandame, Pauline [Auteur]
Laboratoire de Régulation des Signaux de Division
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Spriet, Corentin [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Trinel, Dave [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Gelaude, Armance [Auteur]
Laboratoire de Régulation des Signaux de Division
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Cailliau, Katia [Auteur]
Laboratoire de Régulation des Signaux de Division
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Bompard, Coralie [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Biondi, Emanuele G. [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Bodart, Jean-Francois [Auteur]
Laboratoire de Régulation des Signaux de Division
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Laboratoire de Régulation des Signaux de Division
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Spriet, Corentin [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Trinel, Dave [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Gelaude, Armance [Auteur]
Laboratoire de Régulation des Signaux de Division
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Cailliau, Katia [Auteur]
Laboratoire de Régulation des Signaux de Division
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Bompard, Coralie [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Biondi, Emanuele G. [Auteur]
Institut de Recherche Interdisciplinaire [Villeneuve d'Ascq] [IRI]
Bodart, Jean-Francois [Auteur]
Laboratoire de Régulation des Signaux de Division
Site de Recherche Intégrée en Cancérologie [SIRIC-ONCOLille]
Titre de la revue :
Cell Cycle
Numéro :
13
Pagination :
3232-3240
Date de publication :
2014-10-15
ISSN :
1538-4101, 1551-4005
Mot(s)-clé(s) en anglais :
Cell cycle
chromosome segregation
FRET based biosensor
Mitosis
PKA
chromosome segregation
FRET based biosensor
Mitosis
PKA
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
The cyclic adenosine monophosphate dependent kinase protein (PKA) controls a variety of cellular processes including cell cycle regulation. Here, we took advantages of genetically encoded FRET-based biosensors, using an ...
Lire la suite >The cyclic adenosine monophosphate dependent kinase protein (PKA) controls a variety of cellular processes including cell cycle regulation. Here, we took advantages of genetically encoded FRET-based biosensors, using an AKAR-derived biosensor to characterize PKA activity during mitosis in living HeLa cells using a single-cell approach. We measured PKA activity changes during mitosis. HeLa cells exhibit a substantial increase during mitosis, which ends with telophase. An AKAREV T>A inactive form of the biosensor and H89 inhibitor were used to ascertain for the specificity of the PKA activity measured. On a spatial point of view, high levels of activity near to chromosomal plate during metaphase and anaphase were detected. By using the PKA inhibitor H89, we assessed the role of PKA in the maintenance of a proper division phenotype. While this treatment in our hands did not impaired cell cycle progression in a drastic manner, inhibition of PKA leads to a dramatic increase in chromososme misalignement on the spindle during metaphase that could result in aneuploidies. Our study emphasizes the insights that can be gained with genetically encoded FRET-based biosensors, which enable to overcome the shortcomings of classical methologies and unveil in vivo PKA spatiotemporal profiles in HeLa cells.Lire moins >
Lire la suite >The cyclic adenosine monophosphate dependent kinase protein (PKA) controls a variety of cellular processes including cell cycle regulation. Here, we took advantages of genetically encoded FRET-based biosensors, using an AKAR-derived biosensor to characterize PKA activity during mitosis in living HeLa cells using a single-cell approach. We measured PKA activity changes during mitosis. HeLa cells exhibit a substantial increase during mitosis, which ends with telophase. An AKAREV T>A inactive form of the biosensor and H89 inhibitor were used to ascertain for the specificity of the PKA activity measured. On a spatial point of view, high levels of activity near to chromosomal plate during metaphase and anaphase were detected. By using the PKA inhibitor H89, we assessed the role of PKA in the maintenance of a proper division phenotype. While this treatment in our hands did not impaired cell cycle progression in a drastic manner, inhibition of PKA leads to a dramatic increase in chromososme misalignement on the spindle during metaphase that could result in aneuploidies. Our study emphasizes the insights that can be gained with genetically encoded FRET-based biosensors, which enable to overcome the shortcomings of classical methologies and unveil in vivo PKA spatiotemporal profiles in HeLa cells.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
CNRS
Université de Lille
Université de Lille
Équipe(s) de recherche :
Plant Storage Polysaccharides
Régulation des signaux de division
Régulation des signaux de division
Date de dépôt :
2020-02-12T15:45:24Z
2020-04-15T10:38:23Z
2020-04-15T10:40:30Z
2020-04-15T10:38:23Z
2020-04-15T10:40:30Z
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