One, Two, Three: A Bioorthogonal Triple ...
Document type :
Article dans une revue scientifique
DOI :
Permalink :
Title :
One, Two, Three: A Bioorthogonal Triple Labelling Strategy for Studying the Dynamics of Plant Cell Wall Formation In Vivo
Author(s) :
Simon, Clémence [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Lion, Cedric [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Baldacci-Cresp, Fabien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Hawkins, Simon [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Biot, Christophe [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Lion, Cedric [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Spriet, Corentin [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Baldacci-Cresp, Fabien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Hawkins, Simon [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Biot, Christophe [Auteur]

Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Journal title :
Angewandte Chemie International Edition
Volume number :
57
Pages :
16665-16671
Publication date :
2018-12-17
ISSN :
1433-7851
English keyword(s) :
bioimaging
bioorganic chemistry
bioorthogonal chemistry
lignin
plant cell wall
bioorganic chemistry
bioorthogonal chemistry
lignin
plant cell wall
HAL domain(s) :
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Reported herein is an in vivo triple labelling strategy to monitor the formation of plant cell walls. Based on a combination of copper-catalysed alkyne–azide cycloaddition (CuAAC), strain-promoted azide–alkyne cycloaddition ...
Show more >Reported herein is an in vivo triple labelling strategy to monitor the formation of plant cell walls. Based on a combination of copper-catalysed alkyne–azide cycloaddition (CuAAC), strain-promoted azide–alkyne cycloaddition (SPAAC), and Diels–Alder reaction with inverse electronic demand (DARinv), this methodology can be applied to various plant species of interest in research. It allowed detection of the differential incorporation of alkynyl-, azido-, and methylcyclopropenyl-tagged reporters of the three main monolignols into de novo biosynthesized lignin in different tissues, cell types, or cell wall layers. In addition, this triple labelling was implemented with different classes of chemical reporters, using two monolignol reporters in conjunction with alkynylfucose to simultaneously monitor the biosynthesis of lignin and non-cellulosic polysaccharides. This allowed observation of their deposition occurring contemporaneously in the same cell wall.Show less >
Show more >Reported herein is an in vivo triple labelling strategy to monitor the formation of plant cell walls. Based on a combination of copper-catalysed alkyne–azide cycloaddition (CuAAC), strain-promoted azide–alkyne cycloaddition (SPAAC), and Diels–Alder reaction with inverse electronic demand (DARinv), this methodology can be applied to various plant species of interest in research. It allowed detection of the differential incorporation of alkynyl-, azido-, and methylcyclopropenyl-tagged reporters of the three main monolignols into de novo biosynthesized lignin in different tissues, cell types, or cell wall layers. In addition, this triple labelling was implemented with different classes of chemical reporters, using two monolignol reporters in conjunction with alkynylfucose to simultaneously monitor the biosynthesis of lignin and non-cellulosic polysaccharides. This allowed observation of their deposition occurring contemporaneously in the same cell wall.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
CNRS
Université de Lille
Université de Lille
Research team(s) :
Fibres végétales
Chemical Glycobiology
Chemical Glycobiology
Submission date :
2020-02-12T15:45:47Z
2021-05-26T07:10:06Z
2021-05-26T07:10:06Z