Title :

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry

Author(s) :

Donnelly, Daniel [Auteur]
Northeastern University [Boston]
Rawlins, Catherine [Auteur]
Northeastern University [Boston]
Dehart, Caroline [Auteur]
Northwestern University [Evanston]
Fornelli, Luca [Auteur]
Northwestern University [Evanston]
Schachner, Luis [Auteur]
Northwestern University [Evanston]
Lin, Ziqing [Auteur]
University of Wisconsin-Madison
Lippens, Jennifer [Auteur]
Applied Molecular Genetics [AMGen]
Aluri, Krishna [Auteur]
Northeastern University [Boston]
Sarin, Richa [Auteur]
Northeastern University [Boston]
Chen, Bifan [Auteur]
University of Wisconsin-Madison
Lantz, Carter [Auteur]
University of California [Los Angeles] [UCLA]
Jung, Wonhyeuk [Auteur]
University of California [Los Angeles] [UCLA]
Johnson, Kendall [Auteur]
Northeastern University [Boston]
Koller, Antonius [Auteur]
Northeastern University [Boston]
Wolff, Jeremy [Auteur]
Campuzano, Iain [Auteur]
Applied Molecular Genetics [AMGen]
Auclair, Jared [Auteur]
Northeastern University [Boston]
Ivanov, Alexander [Auteur]
Northeastern University [Boston]
Whitelegge, Julian [Auteur]
University of California [Los Angeles] [UCLA]
Paša-Tolić, Ljiljana [Auteur]
Pacific Northwest National Laboratory [PNNL]
Chamot-Rooke, Julia [Auteur]
Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology [UTechS MSBio]
Danis, Paul [Auteur]
Smith, Lloyd [Auteur]
University of Wisconsin-Madison
Tsybin, Yury [Auteur]
Spectroswiss [Lausanne, Switzerland]
Loo, Joseph [Auteur]
University of California [Los Angeles] [UCLA]
Ge, Ying [Auteur]
University of Wisconsin-Madison
Kelleher, Neil [Auteur]
Northwestern University [Evanston]
Agar, Jeffrey [Auteur correspondant]
Northeastern University [Boston]

Journal title :

Nature Methods

Pages :

587-594

Publisher :

Nature Publishing Group

Publication date :

2019-07

ISSN :

1548-7091

HAL domain(s) :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire

English abstract : [en]

One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the ...
Show more >One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.Show less >

Language :

Anglais

Popular science :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL