Nonribosomal peptide synthetase with a ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Title :
Nonribosomal peptide synthetase with a unique iterative-alternative-optional mechanism catalyzes amonabactin synthesis in Aeromonas
Author(s) :
Esmaeel, Qassim [Auteur]
Université de Lille
Adaptation au froid du pois [AFP]
Chevalier, Mickael [Auteur]
Université de Lille
Adaptation au froid du pois [AFP]
Chataigné, Gabrielle [Auteur]
Université de Lille
Adaptation au froid du pois [AFP]
Subashkumar, Rathinasamy [Auteur]
Université de Lille
Adaptation au froid du pois [AFP]
Jacques, Philippe [Auteur]
Université de Liège
TERRA Teaching and Research Centre
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Leclere, Valerie [Auteur]
Adaptation au froid du pois [AFP]
Université de Lille
Centre National de la Recherche Scientifique [CNRS]
Bioinformatics and Sequence Analysis [BONSAI]
Université de Lille
Adaptation au froid du pois [AFP]
Chevalier, Mickael [Auteur]
Université de Lille
Adaptation au froid du pois [AFP]
Chataigné, Gabrielle [Auteur]

Université de Lille
Adaptation au froid du pois [AFP]
Subashkumar, Rathinasamy [Auteur]
Université de Lille
Adaptation au froid du pois [AFP]
Jacques, Philippe [Auteur]
Université de Liège
TERRA Teaching and Research Centre
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Leclere, Valerie [Auteur]

Adaptation au froid du pois [AFP]
Université de Lille
Centre National de la Recherche Scientifique [CNRS]
Bioinformatics and Sequence Analysis [BONSAI]
Journal title :
Applied Microbiology And Biotechnology
Pages :
8453-8463
Publisher :
Springer Verlag
Publication date :
2016
ISSN :
0175-7598
English keyword(s) :
Aeromonas
Amonabactin
Nonribosomal peptide synthetase
Siderophore
NRPS
Amonabactin
Nonribosomal peptide synthetase
Siderophore
NRPS
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Based on the exploration of data generated by genome sequencing, a bioinformatics approach has been chosen to identify the biosynthetic pathway of the siderophores produced by Aeromonas species. The amonabactins, considered ...
Show more >Based on the exploration of data generated by genome sequencing, a bioinformatics approach has been chosen to identify the biosynthetic pathway of the siderophores produced by Aeromonas species. The amonabactins, considered as a virulence factor, represent a family of four variants of catechol peptidic siderophores containing Dhb, Lys, Gly, and an aromatic residue either Trp or Phe in a D-configuration. The synthesis operon is constituted of seven genes named amoCEBFAGH and is iron-regulated. The cluster includes genes encoding proteins involved in the synthesis and incorporation of the Dhb monomer, and genes encoding specific nonribosomal peptide synthetases, which are responsible for the building of the peptidic moiety. The amonabactin assembly line displays a still so far not described atypical mode of synthesis that is iterative, alternative, and optional. A disruption mutant in the adenylation domain of AmoG was unable to synthesize any amonabactin and to grow in iron stress conditions while a deletion of amoH resulted in the production of only two over the four forms. The amo cluster is widespread among most of the Aeromonas species, only few species produces the enterobactin siderophore.Show less >
Show more >Based on the exploration of data generated by genome sequencing, a bioinformatics approach has been chosen to identify the biosynthetic pathway of the siderophores produced by Aeromonas species. The amonabactins, considered as a virulence factor, represent a family of four variants of catechol peptidic siderophores containing Dhb, Lys, Gly, and an aromatic residue either Trp or Phe in a D-configuration. The synthesis operon is constituted of seven genes named amoCEBFAGH and is iron-regulated. The cluster includes genes encoding proteins involved in the synthesis and incorporation of the Dhb monomer, and genes encoding specific nonribosomal peptide synthetases, which are responsible for the building of the peptidic moiety. The amonabactin assembly line displays a still so far not described atypical mode of synthesis that is iterative, alternative, and optional. A disruption mutant in the adenylation domain of AmoG was unable to synthesize any amonabactin and to grow in iron stress conditions while a deletion of amoH resulted in the production of only two over the four forms. The amo cluster is widespread among most of the Aeromonas species, only few species produces the enterobactin siderophore.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Non spécifiée
Popular science :
Non
Collections :
Source :