Title :

In situ microscopic cytometry enables noninvasive viability assessment of animal cells by measuring entropy states

Author(s) :

Wiedemann, Philipp [Auteur correspondant]
Guez, Jean Sébastien [Auteur correspondant]
Procédés Biologiques, Génie Enzymatique et Microbien - EA1026 [ProBioGEM]
Wiegemann, Hans B. [Auteur]
Egner, Florian [Auteur]
Quintana, Juan C. [Auteur]
Department of Computer and Information Science, University of Konstanz
Asanza-Maldonado, Diego [Auteur]
Filipaki, Marcos [Auteur]
Wilkesman, Jeff [Auteur]
Schwiebert, Christian [Auteur]
Cassar, Jean Philippe [Auteur]
Systèmes Tolérants aux Fautes [STF]
Dhulster, P. [Auteur]
Procédés Biologiques, Génie Enzymatique et Microbien - EA1026 [ProBioGEM]
Suhr, H. [Auteur]

Journal title :

Biotechnology and bioengineering

Pages :

2884-2893

Publisher :

Wiley

Publication date :

2011-12

ISSN :

0006-3592

HAL domain(s) :

Sciences de l'ingénieur [physics]/Automatique / Robotique
Sciences du Vivant [q-bio]/Biotechnologies
Informatique [cs]/Biotechnologie

English abstract : [en]

Current state of the art to determine the viability of animal cell suspension cultures is based on sampling and subsequent counting using specific staining assays. We demonstrate for the first time a noninvasive in situ ...
Show more >Current state of the art to determine the viability of animal cell suspension cultures is based on sampling and subsequent counting using specific staining assays. We demonstrate for the first time a noninvasive in situ imaging cytometry capable of determining the statistics of a morphologic transition during cell death in suspension cultures. To this end, we measure morphometric inhomogeneity--defined as information entropy--in cell in situ micrographs. We found that the cells are partitioned into two discrete entropy states broadened by phenotypical variability. During the normal course of a culture or by inducing cell death, we observe the transition of cells between these states. As shown by comparison with ex situ diagnostics, the entropy transition happens before or while the cytoplasmatic membrane is loosing its ability to exclude charged dyes. Therefore, measurement of morphometric inhomogeneity constitutes a noninvasive assessment of viability in real time. Biotechnol.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL