Title :

Protein Sequencing with an Adaptive Genetic Algorithm from Tandem Mass Spectrometry

Author(s) :

Boisson, Jean-Charles [Auteur correspondant]
Parallel Cooperative Multi-criteria Optimization [DOLPHIN]
Laboratoire d'Informatique Fondamentale de Lille [LIFL]
Jourdan, Laetitia [Auteur]
Parallel Cooperative Multi-criteria Optimization [DOLPHIN]
Laboratoire d'Informatique Fondamentale de Lille [LIFL]
Talbi, El-Ghazali [Auteur]
Parallel Cooperative Multi-criteria Optimization [DOLPHIN]
Rolando, Christian [Auteur]
Laboratoire de Chimie Organique et Macromoleculaire [UMR CNRS 8009]

Conference title :

CEC 2006

City :

Vancouver

Country :

Canada

Start date of the conference :

2006-07-16

Publication date :

2006

English keyword(s) :

Proteomics
protein sequencing
tandem mass spectrometry

HAL domain(s) :

Informatique [cs]/Bio-informatique [q-bio.QM]
Sciences du Vivant [q-bio]/Bio-Informatique, Biologie Systémique [q-bio.QM]

English abstract : [en]

In Proteomics, only the de novo peptide sequencing approach allows a partial amino acid sequence of a peptide to be found from a MS/MS spectrum. In this article a preliminary work is presented to discover a complete protein ...
Show more >In Proteomics, only the de novo peptide sequencing approach allows a partial amino acid sequence of a peptide to be found from a MS/MS spectrum. In this article a preliminary work is presented to discover a complete protein sequence from spectral data (MS and MS/MS spectra). For the moment, our approach only uses MS spectra. A Genetic Algorithm (GA) has been designed with a new evaluation function which works directly with a complete MS spectrum as input and not with a mass list like the other methods using this kind of data. Thus the mono isotopic peak extraction step which needs a human intervention is deleted. The goal of this approach is to discover the sequence of unknown proteins and to allow a better understanding of the differences between experimental proteins and proteins from databases.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre de Recherche en Informatique, Signal et Automatique de Lille (CRIStAL) - UMR 9189

Source :

Harvested from HAL