Title :

Virus and cystic fibrosis: Rhinoviruses are associated with exacerbations in adult patients

Author(s) :

Goffard, Anne [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Lambert, Valérie [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Salleron, Julia [Auteur]
Institut de Cancérologie de Lorraine - Alexis Vautrin [Nancy] [UNICANCER/ICL]
Herwegh, Stéphanie [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Engelmann, Ilka [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Pinel, Claudine [Auteur]
Centre Hospitalier Universitaire [Grenoble] [CHU]
Pin, Isabelle [Auteur]
Centre Hospitalier Universitaire [Grenoble] [CHU]
Perrez, Thierry [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Prévotat, Anne [Auteur]
Hôpital Albert Calmette
Dewilde, Anny [Auteur]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]
Delhaes, Laurence [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Centre Hospitalier Régional Universitaire [Lille] [CHRU Lille]

Journal title :

Journal of Clinical Virology

Pages :

147-153

Publisher :

Elsevier

Publication date :

2014-06

ISSN :

1386-6532

English keyword(s) :

PCR
Pulmonary exacerbation
Rhinovirus
Cystic fibrosis

HAL domain(s) :

Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie
Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieuses

English abstract : [en]

BackgroundFew studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated.MethodSixty-four sputum samples collected from 46 adult ...
Show more >BackgroundFew studies have suggested the potential role of respiratory viruses in cystic fibrosis (CF) exacerbation, but their real impact is probably underestimated.MethodSixty-four sputum samples collected from 46 adult patients were included in the study: 33 samples were collected during exacerbation of CF, and 31 during the stable phase. After extraction, nucleic acids were tested for the presence of respiratory viruses. When rhinovirus (HRV) was detected, the 5′UTR and VP4/2 regions were sequenced, and phylogenetically analyzed. The characteristics of patients in exacerbation and stable phase were compared.ResultsViruses were found in 25% of samples. The HRV viruses were the most frequently detected followed by coronaviruses. Only the HRV detection was significantly associated with the occurrence of CF pulmonary exacerbation (p < 0.027). Characterization of 5′UTR and VP4/2 regions of the HRV genome specified that HRV-A, -B, -C were detected. All HRV-C were recombinant HRV-Ca.ConclusionsHRV were the most frequently detected viruses; their detection was significantly associated with the occurrence of an exacerbation. The reality of viral recombination between HRV was demonstrated in CF patients for the first time, raising the role of viruses in lung microbiota. Further studies are now warranted to decipher virus impact in CFShow less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL