Title :

Heterogeneity of subspecies Mycobacterium avium paratuberculosis from genotype to phenotype

Author(s) :

Lefrançois, Louise [Auteur]
Infectiologie Animale et Santé Publique [UR IASP]
Bodier, Christelle [Auteur]
Infectiologie Animale et Santé Publique [UR IASP]
Cochard, Thierry [Auteur]
Infectiologie Animale et Santé Publique [UR IASP]
Gilbert, Florence [Auteur]
Infectiologie Animale et Santé Publique [UR IASP]
Canepa, Sylvie [Auteur]
Physiologie de la reproduction et des comportements [Nouzilly] [PRC]
Lecher, Sophie [Auteur]
Université Lille Nord de France (COMUE)
Institut Pasteur [Paris] [IP]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Raze, Dominique [Auteur]
Université Lille Nord de France (COMUE)
Institut Pasteur [Paris] [IP]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Haguenoer, Eve [Auteur]
Centre Hospitalier Régional Universitaire de Tours [CHRU Tours]
Lanotte, Philippe [Auteur]
Université de Tours [UT]
Sevilla, Iker A. [Auteur]
Instituto Vasco de Investigación y Desarrollo Agrario [Derio] [NEIKER]
Stevenson, Karen [Auteur]
Moredun Research Institute [Penicuik, UK] [MRI]
Locht, Camille [Auteur]
Infectiologie Animale et Santé Publique [UR IASP]
Biet, Franck [Auteur]
Infectiologie Animale et Santé Publique [UR IASP]

Conference title :

2011 PhD Day

City :

Nouzilly

Country :

France

Start date of the conference :

2011-10-07

Publication date :

2011

English keyword(s) :

Mycobacterium avium paratuberculosis

HAL domain(s) :

Sciences du Vivant [q-bio]
Informatique [cs]

English abstract : [en]

Background - In the subspecies M avium ssp. paratuberculosis (Map) two groups, known as Cattle (C) and Sheep (S), have been defined by genotyping. Recent studies show that Map C and S have different phenotypes with respect ...
Show more >Background - In the subspecies M avium ssp. paratuberculosis (Map) two groups, known as Cattle (C) and Sheep (S), have been defined by genotyping. Recent studies show that Map C and S have different phenotypes with respect to infection of macrophages and iron metabolism. Map is adapted to the gastrointestinal tract of ruminant, but the mechanism of entry is currently unknown. In this study, we investigated the phenotype of the Map-host interaction, involving the virulence factor Heparin-Binding HemAgglutinin (HBHA), for both groups of Map. Method - A large collection of Map isolates (types C & S) genotyped were used to investigate the polymorphism of the hbhA gene by GeneMapper. The biochemical features of HBHA produced by these two types were analyzed by Heparin-Sepharose Chromatography (HSC) and Surface Plasmon Resonance (SPR). Results - In silico analyses of both types of Map have revealed two forms of hbhA. This observation, showing that hbhA is distinct according to the group, was confirmed using GeneMapper on 94 Map strains (76 Map C & 18 MapS) with various genotypes. We found that Map C produces HBHA with a short C-terminal domain, while that of type S presents a long C-terminal domain, similar to that of HBHA produced by M. tuberculosis. The purification of recombinant HBHA from Map C and S by HSC, highlighted a correlation between their affinities to heparin and the length of their C-terminal domain, which has been quantified by SPR. Conclusion- We show for the first time that the types C and S of Map may be distinguished by the type of HBHA they produce. Thus, HBHA participates in the genotypic and phenotypic differences observed between the C and S types of Map.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Nationale

Popular science :

Non

Collections :

• Centre d'Infection et d'Immunité de Lille (CIIL) - U1019 - UMR 9017

Source :

Harvested from HAL