Titre :

Methylation-dependent transcriptional regulation of crescentin gene (creS) by GcrA in Caulobacter crescentus.

Auteur(s) :

Mohapatra, Saswat Sourav [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Fioravanti, Antonella [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Vandame, Pauline [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Pini, Francesco [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bompard, Coralie [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Blossey, Ralf [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Valette, Odile [Auteur]
Laboratoire de chimie bactérienne [LCB]
Biondi, Emanuele G [Auteur]
Laboratoire de chimie bactérienne [LCB]

Titre de la revue :

Molecular Microbiology

Nom court de la revue :

Mol Microbiol

Numéro :

114

Pagination :

127-139

Date de publication :

2020-03-18

ISSN :

1365-2958

Mot(s)-clé(s) en anglais :

Caulobacter crescentus
CcrM
DNA methylation
GcrA
transcription

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Immunologie/Allergologie

Résumé en anglais : [en]

In Caulobacter crescentus the combined action of chromosome replication and the expression of DNA methyl-transferase CcrM at the end of S-phase maintains a cyclic alternation between a full- to hemi-methylated chromosome. ...
Lire la suite >In Caulobacter crescentus the combined action of chromosome replication and the expression of DNA methyl-transferase CcrM at the end of S-phase maintains a cyclic alternation between a full- to hemi-methylated chromosome. This transition of the chromosomal methylation pattern affects the DNA-binding properties of the transcription factor GcrA that controls the several key cell cycle functions. However, the molecular mechanism by which GcrA and methylation are linked to transcription is not fully elucidated yet. Using a combination of cell biology, genetics, and in vitro analysis, we deciphered how GcrA integrates the methylation pattern of several S-phase expressed genes to their transcriptional output. We demonstrated in vitro that transcription of ctrA from the P1 promoter in its hemi-methylated state is activated by GcrA, while in its fully methylated state GcrA had no effect. Further, GcrA and methylation together influence a peculiar distribution of creS transcripts, encoding for crescentin, the protein responsible for the characteristic shape of Caulobacter cells. This gene is duplicated at the onset of chromosome replication and the two hemi-methylated copies are spatially segregated. Our results indicated that GcrA transcribed only the copy where coding strand is methylated. In vitro transcription assay further substantiated this finding. As several of the cell cycle-regulated genes are also under the influence of methylation and GcrA-dependent transcriptional regulation, this could be a mechanism responsible for maintaining the gene transcription dosage during the S-phase.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Non spécifiée

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Plant Storage Polysaccharides
Computational Molecular Systems Biology

Plateforme(s) de recherche :

Traitement de l'image et du signal pour la biologie (TISBio)

Date de dépôt :

2020-07-06T14:23:52Z
2020-09-28T10:23:38Z
2020-11-27T13:03:47Z
2021-12-09T11:42:17Z