Titre :

Measuring Interactions between Fluorescent Probes and Lignin in Plant Sections by sFLIM Based on Native Autofluorescence

Auteur(s) :

Terryn, Christine [Auteur]
Plateforme en Imagerie Cellulaire et Tissulaire [PICT]
Habrant, Anouck [Auteur]
Fractionnement des AgroRessources et Environnement [FARE]
Paës, Gabriel [Auteur]
Fractionnement des AgroRessources et Environnement [FARE]
Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Titre de la revue :

Journal of Visualized Experiments

Nom court de la revue :

JoVE

Éditeur :

MyJove Corporation

Date de publication :

2020-01-02

ISSN :

1940-087X

Mot(s)-clé(s) en anglais :

Biochemistry
Issue 155
sFLIM
Fluorescence lifetime
FRET
Autofluorescence
Lignocellulose
Interaction

Discipline(s) HAL :

Chimie

Résumé en anglais : [en]

In lignocellulosic biomass (LB), the activity of enzymes is limited by the appearance of non-specific interactions with lignin during the hydrolysisprocess, which maintains enzymes far from their substrate. Characterization ...
Lire la suite >In lignocellulosic biomass (LB), the activity of enzymes is limited by the appearance of non-specific interactions with lignin during the hydrolysisprocess, which maintains enzymes far from their substrate. Characterization of these complex interactions is thus a challenge in complexsubstrates such as LB. The method here measures molecular interactions between fluorophore-tagged molecules and native autofluorescentlignin, to be revealed by Förster resonance energy transfer (FRET). Contrary to FRET measurements in living cells using two exogenousfluorophores, FRET measurements in plants using lignin is not trivial due to its complex autofluorescence. We have developed an originalacquisition and analysis pipeline with correlated observation of two complementary properties of fluorescence: fluorescence emission andlifetime. sFLIM (spectral and fluorescent lifetime imaging microscopy) provides the quantification of these interactions with high sensitivity,revealing different interaction levels between biomolecules and lignin.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Non spécifiée

Projet ANR :

Modélisation de la progression d’enzymes dans des assemblages et parois lignocellulosiques

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Plateforme(s) de recherche :

Traitement de l'image et du signal pour la biologie (TISBio)

Date de dépôt :

2020-07-06T14:32:48Z
2020-09-28T10:34:18Z