Titre :

Comparative Study on the Impact of Growth Conditions on the Physiology and the Virulence of Pseudomonas aeruginosa Biofilm and Planktonic Cells

Auteur(s) :

Khelissa, Simon [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Abdallah, Marwan [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Jama, charafeddine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Barras, Alexandre [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Chihib, Nour-Eddine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Unité Matériaux et Transformations (UMET) - UMR 8207

Titre de la revue :

Journal of Food Protection

Numéro :

82

Pagination :

1357-1363

Éditeur :

International Association for Food Protection

Date de publication :

2019-07-17

Discipline(s) HAL :

Chimie/Matériaux
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie

Résumé en anglais : [en]

The aim of the present work was to study and compare the effect of growth temperature (20, 30, and 37°C) and surface type (stainless steel and polycarbonate) on the production of virulence factors, such as proteases and ...
Lire la suite >The aim of the present work was to study and compare the effect of growth temperature (20, 30, and 37°C) and surface type (stainless steel and polycarbonate) on the production of virulence factors, such as proteases and siderophores, and the risk of surface contamination associated with Pseudomonas aeruginosa biofilm and planktonic cells. The increase of growth temperature from 20 to 37°C increased (approximately twofold) the electronegative charge and the hydrophobicity of the P. aeruginosa biofilm cell surface. P. aeruginosa biofilm cell adhesion to stainless steel and polycarbonate was 5- and 1.5-fold higher than their planktonic counterparts at 20 and 30°C, respectively. The increase of growth temperature from 20 to 37°C increased the production of proteases (twofold) and siderophores (twofold) and the cytotoxicity (up to 30-fold) against the HeLa cell line in the supernatants of P. aeruginosa planktonic and biofilm cultures. This study also highlighted that biofilm and planktonic P. aeruginosa cells exhibited distinct physiological properties with respect to the production of virulence factors and the cytotoxicity against the Hela cell line. Therefore, effective disinfection procedures should be adapted to inactivate bacteria detached from biofilms.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Équipe(s) de recherche :

Ingénierie des Systèmes Polymères
Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Date de dépôt :

2020-07-09T17:23:11Z
2020-08-26T09:06:37Z
2021-05-25T12:34:08Z