Colorimetric sensing of dopamine in beef ...
Type de document :
Article dans une revue scientifique
URL permanente :
Titre :
Colorimetric sensing of dopamine in beef meat using copper sulfide encapsulated within bovine serum albumin functionalized with copper phosphate (CuS-BSA-Cu3(PO4)2) nanoparticles
Auteur(s) :
Swaidan, Abir [Auteur]
Barras, Alexandre [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Addad, Ahmed [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Tahon, Jean-Francois [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Toufaily, Joumana [Auteur]
Hamieh, Tayssir [Auteur]
Szunerits, Sabine [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Boukherroub, Rabah [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Barras, Alexandre [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Addad, Ahmed [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Tahon, Jean-Francois [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Toufaily, Joumana [Auteur]
Hamieh, Tayssir [Auteur]
Szunerits, Sabine [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Boukherroub, Rabah [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Titre de la revue :
Journal of Colloid and Interface Science
Nom court de la revue :
Journal of Colloid and Interface Science
Pagination :
732-740
Éditeur :
Elsevier BV
Date de publication :
2021-01
ISSN :
0021-9797
Mot(s)-clé(s) en anglais :
CuS-BSA-Cu3(PO4)2
Nanozymes
Colorimetric
Detection
Dopamine
Beef meat
Nanozymes
Colorimetric
Detection
Dopamine
Beef meat
Discipline(s) HAL :
Chimie/Chimie théorique et/ou physique
Résumé en anglais : [en]
A novel nanosensor with peroxidase enzyme-mimetic activity, based on CuS-BSA-Cu3(PO4)2 nanoparticles, was developed. CuS-BSA nanoparticles were first synthesized using a facile bio-mineralization assay. Conjugation of ...
Lire la suite >A novel nanosensor with peroxidase enzyme-mimetic activity, based on CuS-BSA-Cu3(PO4)2 nanoparticles, was developed. CuS-BSA nanoparticles were first synthesized using a facile bio-mineralization assay. Conjugation of Cu3(PO4)2 with CuS-BSA generates CuS-BSA-Cu3(PO4)2 nanoparticles (NPs) of 10 nm in size with high catalytic activity against a peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). The catalytic action was based on a remarkable color change from colorless TMB into blue oxidized product (oxTMB) with absorption maximum at 654 nm. The enzyme-mimetic activity of CuS-BSA-Cu3(PO4)2 nanoparticles was believed to occur through hydroxyl radical (HOradical dot) generation in presence of H2O2, which was inhibited upon addition of dopamine. Increasing concentrations of dopamine induced a gradual decrease of the nanoparticles’ catalytic activity. The developed colorimetric sensor displayed a limit of detection of 0.13 µM for dopamine over 0.05–100 µM linear range and high specificity. The performance of the nanosensor for sensing dopamine in beef meat and blood samples was evaluated and proved to be promising for diagnostic applications without the requirement of complex and expensive instrumentation.Lire moins >
Lire la suite >A novel nanosensor with peroxidase enzyme-mimetic activity, based on CuS-BSA-Cu3(PO4)2 nanoparticles, was developed. CuS-BSA nanoparticles were first synthesized using a facile bio-mineralization assay. Conjugation of Cu3(PO4)2 with CuS-BSA generates CuS-BSA-Cu3(PO4)2 nanoparticles (NPs) of 10 nm in size with high catalytic activity against a peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). The catalytic action was based on a remarkable color change from colorless TMB into blue oxidized product (oxTMB) with absorption maximum at 654 nm. The enzyme-mimetic activity of CuS-BSA-Cu3(PO4)2 nanoparticles was believed to occur through hydroxyl radical (HOradical dot) generation in presence of H2O2, which was inhibited upon addition of dopamine. Increasing concentrations of dopamine induced a gradual decrease of the nanoparticles’ catalytic activity. The developed colorimetric sensor displayed a limit of detection of 0.13 µM for dopamine over 0.05–100 µM linear range and high specificity. The performance of the nanosensor for sensing dopamine in beef meat and blood samples was evaluated and proved to be promising for diagnostic applications without the requirement of complex and expensive instrumentation.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
Université de Lille
CNRS
INRA
ENSCL
CNRS
INRA
ENSCL
Collections :
Date de dépôt :
2020-11-05T16:27:24Z
2020-11-17T09:04:10Z
2020-11-17T09:04:10Z