Titre :

FRET-Based Enzyme Activity Reporter: Practical Hints for Kinases as Indicators of Virulence

Auteur(s) :

Spriet, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Kasprowicz, Angelina [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Trinel, Dave [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Bodart, Jean-Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Titre de l’ouvrage :

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

Éditeur :

InTech

Date de publication :

2018-03-21

ISBN :

978-953-51-3916-4

Mot(s)-clé(s) en anglais :

genetically encoded biosensor
KAR
fret
MAPK
ERK

Discipline(s) HAL :

Chimie/Chimie théorique et/ou physique

Résumé en anglais : [en]

Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes ...
Lire la suite >Modulation of protein kinases activity is often requested for pathogenicity or virulence. This chapter provides several hints for one who might be interested in using FRET-based kinase activity reporters. The archetypes of these reporters, which are now within the arsenal of biosensors, were devoted to the detection and characterization of the activity of the cAMP-Protein kinase A pathway. Based on the principle of this biosensor, other FRET-based kinase activity reporters emerged. Here, the choice of the kinase to be monitored, the artifacts that might be met, and the flexibility and amenability of the FRET-based kinase activity reporters both for high-throughput analysis and dissection of protein kinase functions are discussed.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Équipe(s) de recherche :

Régulation des signaux de division

Date de dépôt :

2020-11-12T15:41:10Z
2020-11-16T13:38:57Z
2021-01-07T10:58:37Z