Titre :

Multiscale quantitative characterization of demineralized casein micelles: How the partial excision of nano-clusters leads to the aggregation during rehydration

Auteur(s) :

Nogueira, Marcio [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Ben Harb, Salma [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Schmutz, Marc [Auteur]
Doumert, Bertrand [Auteur]
Institut Michel Eugène Chevreul - FR 2638 [IMEC]
Nasser, Sarah [Auteur]
Derensy, Antoine [Auteur]
Karoui, Romdhane [Auteur]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Delaplace, Guillaume [Auteur]
Unité Matériaux et Transformations (UMET) - UMR 8207
Unité Matériaux et Transformations - UMR 8207 [UMET]
Peres De Sa Peixoto Jr, Paulo [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]

Titre de la revue :

Food Hydrocolloids

Nom court de la revue :

Food Hydrocolloids

Numéro :

105

Pagination :

105778

Éditeur :

Elsevier BV

Date de publication :

2020-08

ISSN :

0268-005X

Mot(s)-clé(s) en anglais :

Casein micelles
Demineralization
Structure
Aggregation
Hydrophobic bonds

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Ingénierie des aliments

Résumé en anglais : [en]

The amount of colloidal calcium phosphate (CCP) nanoclusters modifies the structure and functional properties of the casein micelles. This study aims to analyze the structural changes in partially demineralized casein ...
Lire la suite >The amount of colloidal calcium phosphate (CCP) nanoclusters modifies the structure and functional properties of the casein micelles. This study aims to analyze the structural changes in partially demineralized casein micelles (D-CMs) and compare them with native casein micelles (CMs) in order to provide new insights towards the subsequent changes in rehydration behavior. Two rehydration strategies were applied. First is the fast rehydration process (50 °C/1 h), close to the industrial practice, while the second one (25 °C/24 h), a slow rehydration process, used as control. The presence of large (>10 μm) and stirring resistant aggregates in D-CMs were evidenced by Static Light Scattering, being significantly higher for D-CMs rehydrated at 50 °C/1 h. As evidence from the electrophoresis results, the non-covalent interactions play a major role in aggregate formation. The decrease in the CCP concentration (−15%) leads to a more loosely packed/porous structure of D-CMs (Transmission Electronic Microscopy/Cryo-Fracture), and (as observed using Nuclear Magnetic Resonance and Small Angle X-ray Scattering) the demineralization also contributes to increase disorder in the structure of the micelle. Fluorescence Spectroscopy data reveals the presence of casein-tryptophan residues in a more hydrophobic environment shedding light on the importance of hydrophobic interactions for the aggregation of D-CMs. In summary, the study sketches a clear picture of internal rearrangement of CMs structures, in partially demineralized conditions, followed by fast rehydration at high temperatures.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Équipe(s) de recherche :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Date de dépôt :

2020-12-08T14:14:46Z
2020-12-08T14:20:20Z