Title :

Evaluation of the hydrophobic properties of latex microspheres and Bacillus spores. Influence of the particle size on the data obtained by the MATH method (microbial adhesion to hydrocarbons)

Author(s) :

Faille, Christine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Lemy, Christelle [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Allion-Maurer, Audrey [Auteur]
Zoueshtiagh, Farzam [Auteur]
Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]

Journal title :

Colloids and Surfaces B: Biointerfaces

Abbreviated title :

Colloids and Surfaces B: Biointerfaces

Volume number :

182

Pages :

110398

Publisher :

Elsevier BV

Publication date :

2019-10-01

ISSN :

0927-7765

English keyword(s) :

Bacillusspores
Particle
Size
Microspheres
Hydrophobicity
MATH
Water
Contact angle

HAL domain(s) :

Sciences du Vivant [q-bio]/Ingénierie des aliments

English abstract : [en]

The current experimental study investigates the influence of latex microsphere particles' size on the assessment of their hydrophilic/hydrophobic character, using the method known as “Microbial Adhesion to Hydrocarbons” ...
Show more >The current experimental study investigates the influence of latex microsphere particles' size on the assessment of their hydrophilic/hydrophobic character, using the method known as “Microbial Adhesion to Hydrocarbons” (MATH). Since bacteria surfaces often change according to the environment in which they find themselves, most of the experiments here were carried out using the calibrated latex microspheres Polybeads® and Yellow-green Fluoresbrite® (Polyscience) microspheres with diameters between 0.2 μm and 4.5 μm. All the beads had a density of ˜1.05 g/cm3. The first set of experiments was performed to adapt the procedure for measurements of water contact angles to microsphere lawns. It was found that all the microspheres tested were hydrophobic, when using a water contact angle of around 110-118°. However, wide differences were observed using the MATH method. The smaller microspheres (0.2 μm, 0.5 μm +/- 0.75 μm) exhibited a poor affinity to hexadecane, even after long contact times, suggesting a hydrophilic character. In contrast, larger microspheres quickly adhered to hexadecane, which is consistent with the values obtained for the water contact angles observed. These results suggest that, at least where hydrophobic particles are concerned, the MATH method is not suitable for the assessment of the hydrophobic character of particles with diameters of less than 1.0 μm. We lastly investigated whether the data obtained for Bacillus spores could also be affected by spore size. The hydrophobicity of spores of eight Bacillus strains was analysed by both MATH and contact angle. Some discrepancies were observed between both methods but could not be related their size (length or width).Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2020-12-14T15:42:55Z