Title :

Identification of Key Residues in Proteins Through Centrality Analysis and Flexibility Prediction with RINspector

Author(s) :

Brysbaert, Guillaume [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Mauri, Theo [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
De Ruyck, Jerome [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Lensink, Marc [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]

Journal title :

Current Protocols in Bioinformatics

Volume number :

65

Pages :

e66

Publisher :

Wiley

Publication date :

2018-11-29

ISSN :

1934-3396

English keyword(s) :

protein structure
residue interaction network
centrality analysis
flexibility prediction

HAL domain(s) :

Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique

English abstract : [en]

Protein structures inherently contain information as to decipher their functions but the exploitation of this knowledge is not trivial. We recently developed an app for the Cytoscape network visualization and analysis ...
Show more >Protein structures inherently contain information as to decipher their functions but the exploitation of this knowledge is not trivial. We recently developed an app for the Cytoscape network visualization and analysis program, called RINspector, the goal of which was to integrate two different approaches that identify key residues in a protein structure or complex. The first approach consists of calculating centralities on a residue interaction network (RIN) generated from the three-dimensional structure, the second one consists of predicting backbone flexibility and needs only the primary sequence. The identified residues highly correlate with functional relevance and constitute a good set of targets for mutagenesis experiments. Here we present a protocol that details step-by-step how to create a RIN from a structure, and then to calculate centralities and predict flexibilities. We also indicate how to understand and use the results of the analyses.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Computational Molecular Systems Biology

Submission date :

2021-01-04T10:24:21Z
2021-01-07T10:44:42Z
2021-02-08T14:40:03Z