Propriétés anti-inflammatoires des oxylipines ...
Type de document :
Autre communication scientifique (congrès sans actes - poster - séminaire...)
Titre :
Propriétés anti-inflammatoires des oxylipines dérivées du DHA
Auteur(s) :
Bosviel, Rémy [Auteur]
Unité de Nutrition Humaine [UNH]
Joumard-Cubizolles, Laurie [Auteur]
Unité de Nutrition Humaine [UNH]
Chinetti-Gbaguidi, Giulia [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Institut de Recherche sur le Cancer et le Vieillissement [IRCAN]
Bayle, Dominique [Auteur]
Unité de Nutrition Humaine [UNH]
Copin, Corinne [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Hennuyer, Nathalie [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Staels, Bart [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Zanoni, Giuseppe [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Porta, A. [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Balas, Laurence [Auteur]
Université de Montpellier [UM]
Galano, Jean-Marie [Auteur]
Université de Montpellier [UM]
Oger, Camille [Auteur]
Université de Montpellier [UM]
Mazur, André [Auteur]
Unité de Nutrition Humaine [UNH]
Durand, Thierry [Auteur]
Université de Montpellier [UM]
Gladine, Cécile []
Unité de Nutrition Humaine [UNH]
Unité de Nutrition Humaine [UNH]
Joumard-Cubizolles, Laurie [Auteur]
Unité de Nutrition Humaine [UNH]
Chinetti-Gbaguidi, Giulia [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Institut de Recherche sur le Cancer et le Vieillissement [IRCAN]
Bayle, Dominique [Auteur]
Unité de Nutrition Humaine [UNH]
Copin, Corinne [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Hennuyer, Nathalie [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Staels, Bart [Auteur]
Récepteurs nucléaires, maladies cardiovasculaires et diabète - U 1011 [RNMCD]
Institut Européen de Génomique du Diabète - European Genomic Institute for Diabetes - FR 3508 [EGID]
Zanoni, Giuseppe [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Porta, A. [Auteur]
Università degli Studi di Pavia = University of Pavia [UNIPV]
Balas, Laurence [Auteur]
Université de Montpellier [UM]
Galano, Jean-Marie [Auteur]
Université de Montpellier [UM]
Oger, Camille [Auteur]
Université de Montpellier [UM]
Mazur, André [Auteur]
Unité de Nutrition Humaine [UNH]
Durand, Thierry [Auteur]
Université de Montpellier [UM]
Gladine, Cécile []
Unité de Nutrition Humaine [UNH]
Titre de la manifestation scientifique :
9. Journée Scientifique du CRNH Auvergne
Organisateur(s) de la manifestation scientifique :
Centre de Recherche en Nutrition Humaine (CRNH). FRA.
Ville :
Clermont-Ferrand
Pays :
France
Date de début de la manifestation scientifique :
2016-11-24
Date de publication :
2016
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Alimentation et Nutrition
Résumé en anglais : [en]
Whereas the anti-inflammatory properties and mechanisms of action of long chain. 3 PUFAs have been abundantly investigated, research gaps remain regarding the respective contribution and mechanisms of action of their ...
Lire la suite >Whereas the anti-inflammatory properties and mechanisms of action of long chain. 3 PUFAs have been abundantly investigated, research gaps remain regarding the respective contribution and mechanisms of action of their oxygenated metabolites collectively known as oxylipins. A dose-dependent and comparative study was conducted using human primary macrophages. The aim was to compare the anti-inflammatory activity of two types of DHA- derived oxylipins including protectins (NPD1 and PDX), formed through lipoxygenase pathway and the neuroprostanes (14-A4t- and 4-F4t-NeuroP) formed through free-radical mediated oxygenation and suspected to be new anti-inflammatory mediators. Considering the potential ability of these lipid mediators to bind PPARs and knowing the central role of PPARs in the regulation of macrophage inflammatory response, transactivation assays was performed to compare the ability of protectins and neuroprostanes to activate PPARs. All molecules significantly reduced LPS-stimulated expression of cytokines but not at the same doses. Notably, NPD1 showed the most effect at 0.1 µM (IL-6:-14.9%, p<0.05 and TNF-α:-26.7%, p<0.05) while the other oxylipins had greater effects at 10 µM, with the strongest result obtained with the cyclopentenone neuroprostane 14-A4t-NeuroP (IL-6:-49.8%, p<0.001 and TNF-α:-40.8%, p<0.001, respectively). Concerning their binding to PPARs, Neuroprostanes and notably 14-A4t-NeuroP preferentially activate PPARγ while Protectins, especially PDX mainly activate PPARα. Combined together, these results bring new insights to the understanding of the role and mechanisms of action of DHA-derived oxylipins in the regulation of the macrophage inflammatory responseLire moins >
Lire la suite >Whereas the anti-inflammatory properties and mechanisms of action of long chain. 3 PUFAs have been abundantly investigated, research gaps remain regarding the respective contribution and mechanisms of action of their oxygenated metabolites collectively known as oxylipins. A dose-dependent and comparative study was conducted using human primary macrophages. The aim was to compare the anti-inflammatory activity of two types of DHA- derived oxylipins including protectins (NPD1 and PDX), formed through lipoxygenase pathway and the neuroprostanes (14-A4t- and 4-F4t-NeuroP) formed through free-radical mediated oxygenation and suspected to be new anti-inflammatory mediators. Considering the potential ability of these lipid mediators to bind PPARs and knowing the central role of PPARs in the regulation of macrophage inflammatory response, transactivation assays was performed to compare the ability of protectins and neuroprostanes to activate PPARs. All molecules significantly reduced LPS-stimulated expression of cytokines but not at the same doses. Notably, NPD1 showed the most effect at 0.1 µM (IL-6:-14.9%, p<0.05 and TNF-α:-26.7%, p<0.05) while the other oxylipins had greater effects at 10 µM, with the strongest result obtained with the cyclopentenone neuroprostane 14-A4t-NeuroP (IL-6:-49.8%, p<0.001 and TNF-α:-40.8%, p<0.001, respectively). Concerning their binding to PPARs, Neuroprostanes and notably 14-A4t-NeuroP preferentially activate PPARγ while Protectins, especially PDX mainly activate PPARα. Combined together, these results bring new insights to the understanding of the role and mechanisms of action of DHA-derived oxylipins in the regulation of the macrophage inflammatory responseLire moins >
Langue :
Français
Comité de lecture :
Oui
Audience :
Nationale
Vulgarisation :
Non
Commentaire :
Flash Posters : Axe 2
Source :
Fichiers
- https://hal.inrae.fr/hal-02743544/document
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- 2016_Fascicule_%20JS_Clermont_1.pdf
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