A readily usable two‐photon fluorescence ...
Type de document :
Compte-rendu et recension critique d'ouvrage
DOI :
Titre :
A readily usable two‐photon fluorescence lifetime microendoscope
Auteur(s) :
Hage, Charles‐henri [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Leclerc, Pierre [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Marc, Fabert [Auteur]
XLIM [XLIM]
Bardet, Sylvia [Auteur]
BIO-INGENIERIE [XLIM-BIO-INGENIERIE]
Brevier, Julien [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Ducourthial, Guillaume [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Mansuryan, Tigran [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Leray, Aymeric [Auteur]
Kudlinski, Alexandre [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Louradour, Frédéric [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Leclerc, Pierre [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Marc, Fabert [Auteur]
XLIM [XLIM]
Bardet, Sylvia [Auteur]
BIO-INGENIERIE [XLIM-BIO-INGENIERIE]
Brevier, Julien [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Ducourthial, Guillaume [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Mansuryan, Tigran [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Leray, Aymeric [Auteur]
Kudlinski, Alexandre [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Louradour, Frédéric [Auteur]
Photonique Fibre et Sources Cohérentes [XLIM-PHOT]
Titre de la revue :
Journal of Biophotonics
Pagination :
e201800276
Éditeur :
Wiley
Date de publication :
2019-01-02
ISSN :
1864-063X
Mot(s)-clé(s) en anglais :
FLIM
biomedical imaging
fiber optics
nonlinear endoscopy
Ultrashort pulses
biomedical imaging
fiber optics
nonlinear endoscopy
Ultrashort pulses
Discipline(s) HAL :
Sciences du Vivant [q-bio]/Ingénierie biomédicale/Imagerie
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysique
Sciences du Vivant [q-bio]/Biotechnologies
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]/Neurobiologie
Sciences du Vivant [q-bio]/Génétique/Génétique animale
Sciences du Vivant [q-bio]/Biologie du développement/Embryologie et organogenèse
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biophysique
Sciences du Vivant [q-bio]/Biotechnologies
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC]/Neurobiologie
Sciences du Vivant [q-bio]/Génétique/Génétique animale
Sciences du Vivant [q-bio]/Biologie du développement/Embryologie et organogenèse
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biologie moléculaire
Résumé en anglais : [en]
A two-photon fluorescence lifetime (2P-FLIM) microendoscope, capable of energetic metabolism imaging through the intracellular nicotinamide adenine dinucleotide (NADH) autofluorescence, at sub-cellular resolution, is ...
Lire la suite >A two-photon fluorescence lifetime (2P-FLIM) microendoscope, capable of energetic metabolism imaging through the intracellular nicotinamide adenine dinucleotide (NADH) autofluorescence, at sub-cellular resolution, is demonstrated. It exhibits readily usable characteristics such as convenient endoscope probe diameter (≈2 mm), fiber length (>5 m) and data accumulation rate (16 frames per second (fps)), leading to a FLIM refreshing rate of ≈0.1 to 1 fps depending on the sample. The spiral scanning image formation does not influence the instrument response function (IRF) characteristics of the system. Near table-top microscope performances are achieved through a comprehensive system including a home-designed spectro-temporal pulse shaper and a custom air-silica double-clad photonic crystal fiber, which enables to reach up to 40 mW of ≈100 fs pulses @ 760 nm with a 80 MHz repetition rate. A GRadient INdex (GRIN) lens provides a lateral resolution of 0.67 μm at the focus of the fiber probe. Intracellular NADH fluorescence lifetime data are finally acquired on cultured cells at 16 fps.Lire moins >
Lire la suite >A two-photon fluorescence lifetime (2P-FLIM) microendoscope, capable of energetic metabolism imaging through the intracellular nicotinamide adenine dinucleotide (NADH) autofluorescence, at sub-cellular resolution, is demonstrated. It exhibits readily usable characteristics such as convenient endoscope probe diameter (≈2 mm), fiber length (>5 m) and data accumulation rate (16 frames per second (fps)), leading to a FLIM refreshing rate of ≈0.1 to 1 fps depending on the sample. The spiral scanning image formation does not influence the instrument response function (IRF) characteristics of the system. Near table-top microscope performances are achieved through a comprehensive system including a home-designed spectro-temporal pulse shaper and a custom air-silica double-clad photonic crystal fiber, which enables to reach up to 40 mW of ≈100 fs pulses @ 760 nm with a 80 MHz repetition rate. A GRadient INdex (GRIN) lens provides a lateral resolution of 0.67 μm at the focus of the fiber probe. Intracellular NADH fluorescence lifetime data are finally acquired on cultured cells at 16 fps.Lire moins >
Langue :
Anglais
Vulgarisation :
Non
Source :