Title :

Porous silica supports for micro-Raman spectroscopic studies of individual living cells

Author(s) :

Cristini-Robbe, O. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Raulin, K. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Dubart, F. [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Bernard, R. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Kinowski, C. [Auteur]
Laboratoire de Physique des Lasers, Atomes et Molécules - UMR 8523 [PhLAM]
Damene, N. [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
El Yasidi, I. [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Boé, A. [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Turrell, S. [Auteur correspondant]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]

Journal title :

Journal of Molecular Structure

Pages :

232 - 237

Publisher :

Elsevier

Publication date :

2013

ISSN :

0022-2860

English keyword(s) :

Sol-gel
Raman spectroscopy
Silica gels
Living cells
Human breast adenocarcinoma MCF-7 cells

HAL domain(s) :

Chimie/Chimie théorique et/ou physique

English abstract : [en]

A work was undertaken to explore the possibility of using porous silica gels as support materials for in vitro studies of individual living cells. Accordingly, tetra methyl orthosilicate-derived silica xerogels were prepared ...
Show more >A work was undertaken to explore the possibility of using porous silica gels as support materials for in vitro studies of individual living cells. Accordingly, tetra methyl orthosilicate-derived silica xerogels were prepared and tested as substrates for human breast adenocarcinoma MCF-7 cells. Micro-Raman spectra and BET measurements were used to determine the effect of sol-pH and autoclave sterilization on the substrate. MCF-7 cells were then seeded and grown on the resulting silica gels. Combined techniques show that (a) sol-pH affects substrate porosity and (b) autoclave conditions affect only the substrate surface thus increasing the surface porosity. Micro-Raman spectra of <i>in situ</i> grown MCF-7 cells indicate that they proliferate well on these xerogel surfaces with a preference for base-catalyzed substrates and that the surface morphology of supports can have a definite effect on the adherence and on the life time of human cells. Spectral evolutions observed for cells over a period of 48 h have made it possible to show that these substrates enable the easy maintenance of cell viability for over 36 h and that micro-Raman techniques make it possible to study individual cells and to characterize evolutional changes at different stages of their life-times. Results point to these new porous silica supports as promising substrates for rapid screening of drug toxicity.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Non spécifiée

Popular science :

Non

Collections :

• Laboratoire de Physique des Lasers, Atomes et Molécules (PhLAM) - UMR 8523

Source :

Harvested from HAL