Virus-Induced Gene Silencing of Cell Wall ...
Document type :
Partie d'ouvrage: Chapitre
Permalink :
Title :
Virus-Induced Gene Silencing of Cell Wall Genes in Flax (Linum usitatissimum)
Author(s) :
Chantreau, Maxime [Auteur]
Umea Plant Science Center [UPSC]
Neutelings, Godfrey [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Umea Plant Science Center [UPSC]
Neutelings, Godfrey [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Book title :
Virus-Induced Gene Silencing in Plants Methods and Protocols
Pages :
65-74
Publisher :
Springer US
Vincent Courdavault and Sébastien Besseau / Humana Press
Vincent Courdavault and Sébastien Besseau / Humana Press
Publication date :
2020-06-19
English keyword(s) :
VIGS
Flax
Gene silencing
Agroinfiltration
Tobacco rattle virus
Flax
Gene silencing
Agroinfiltration
Tobacco rattle virus
HAL domain(s) :
Sciences du Vivant [q-bio]/Biologie végétale
Chimie/Chimie théorique et/ou physique
Chimie/Chimie théorique et/ou physique
English abstract : [en]
Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived ...
Show more >Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from developmental genes. In the virus-induced gene silencing (VIGS) method, it is possible to take advantage of this mechanism by inserting a plant nucleic fragment within the viral genome to knock down the corresponding gene. This tool has been used in many species as a fast and easy reverse genetics technique in order to gain information on the role of genes with poorly understood functions. Here we describe in detail two Agrobacterium-mediated infection protocols in flax, based on a whole plant vacuum infiltration and a leaf syringe infiltration that systemically impact the transcript levels in the stem.Show less >
Show more >Plants have developed defense mechanisms against viruses by using an RNA silencing-based process, which has many common features with the endogenous RNA silencing pathway used for regulating the level of transcripts derived from developmental genes. In the virus-induced gene silencing (VIGS) method, it is possible to take advantage of this mechanism by inserting a plant nucleic fragment within the viral genome to knock down the corresponding gene. This tool has been used in many species as a fast and easy reverse genetics technique in order to gain information on the role of genes with poorly understood functions. Here we describe in detail two Agrobacterium-mediated infection protocols in flax, based on a whole plant vacuum infiltration and a leaf syringe infiltration that systemically impact the transcript levels in the stem.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Fibres végétales
Submission date :
2021-01-06T16:13:30Z
2021-01-06T16:40:49Z
2021-01-07T07:02:09Z
2021-02-08T15:24:31Z
2021-01-06T16:40:49Z
2021-01-07T07:02:09Z
2021-02-08T15:24:31Z