Title :

The stationary phase regulator CpcR activates cry gene expression in non‐sporulating cells of Bacillus thuringiensis

Author(s) :

Zhang, Ruibin [Auteur]
State Key Laboratory for Biology of Plant Diseases and Insect Pests
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Slamti, Leyla [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Tong, Lei [Auteur]
State Key Laboratory for Biology of Plant Diseases and Insect Pests
Verplaetse, Emilie [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Lemy, Christelle [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]
Peng, Qi [Auteur]
State Key Laboratory for Biology of Plant Diseases and Insect Pests
Guo, Shuyuan [Auteur]
Beijing Institute of Technology [BIT]
Zhang, Jie [Auteur]
State Key Laboratory for Biology of Plant Diseases and Insect Pests
Song, Fuping [Auteur]
State Key Laboratory for Biology of Plant Diseases and Insect Pests
Lereclus, Didier [Auteur]
MICrobiologie de l'ALImentation au Service de la Santé [MICALIS]

Journal title :

Molecular Microbiology

Abbreviated title :

Mol Microbiol

Volume number :

113

Pages :

740-754

Publisher :

Wiley

Publication date :

2019-12-02

English keyword(s) :

Bacillus cereus
division of labour
phenotypic heterogeneity
plasmid
sporulation
transcription

HAL domain(s) :

Sciences du Vivant [q-bio]/Ingénierie des aliments

English abstract : [en]

Cell differentiation within an isogenic population allows the specialisation of subpopulations and a division of labour. Bacillus thuringiensis is a spore‐forming bacterium that produces insecticidal crystal proteins (Cry ...
Show more >Cell differentiation within an isogenic population allows the specialisation of subpopulations and a division of labour. Bacillus thuringiensis is a spore‐forming bacterium that produces insecticidal crystal proteins (Cry proteins) in sporulating cells. We recently reported that strain B. thuringiensis LM1212 presents the unique ability to differentiate into two subpopulations during the stationary phase: spore‐formers and crystal‐producers. Here, we characterised the transcriptional regulator CpcR responsible for this differentiation and the expression of the cry genes. cpcR is located on a plasmid that also harbours cry genes. The alignment of LM1212 cry gene promoters revealed the presence of a conserved DNA sequence upstream from the −35 region. This presumed CpcR box was also found in the promoter of cpcR and we showed that cpcR transcription is positively autoregulated. Electrophoretic mobility shift assays suggested that CpcR directly controls the transcription of its target genes by binding to the CpcR box. We showed that CpcR was able to direct the production of a crystal consisting of a heterologous insecticidal Cry protein in non‐sporulating cells of a typical B. thuringiensis kurstaki strain. Moreover, the expression of cpcR induced a reduction in the sporulation of this B. thuringiensis strain, suggesting an interaction between CpcR and the sporulation regulatory networks.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Université de Lille
CNRS
INRA
ENSCL

Collections :

• Unité Matériaux et Transformations (UMET) - UMR 8207

Research team(s) :

Processus aux Interfaces et Hygiène des Matériaux (PIHM)

Submission date :

2021-01-18T14:06:03Z