Optimised immunofluorescence method on ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Permalink :
Title :
Optimised immunofluorescence method on cleared intact mongolian gerbil cochlea
Author(s) :
Risoud, Michael [Auteur]
Tardivel, M. [Auteur]
Lemesre, Pierre-Emmanuel [Auteur]
Bonne, Nicolas-Xavier [Auteur]
Vincent, Christophe [Auteur]
Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS]
Advanced Drug Delivery Systems (ADDS) - U1008
Tardivel, M. [Auteur]
Lemesre, Pierre-Emmanuel [Auteur]
Bonne, Nicolas-Xavier [Auteur]
Vincent, Christophe [Auteur]

Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS]
Advanced Drug Delivery Systems (ADDS) - U1008
Journal title :
European annals of otorhinolaryngology, head and neck diseases
Abbreviated title :
Eur Ann Otorhinolaryngol Head Neck Dis
Publication date :
2020-02-18
ISSN :
1879-730X
Keyword(s) :
Immunofluorescence
Hair cells
Mongolian gerbil
Laser scanning confocal microscopy
Cochlea
Clarifying
Transparency
Hair cells
Mongolian gerbil
Laser scanning confocal microscopy
Cochlea
Clarifying
Transparency
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Immunofluorescence on cleared intact cochlea allows detailed analysis of the cochlear ultrastructure, while avoiding the problems of dissection and serial sections. Protocols have been developed for mice and Mongolian ...
Show more >Immunofluorescence on cleared intact cochlea allows detailed analysis of the cochlear ultrastructure, while avoiding the problems of dissection and serial sections. Protocols have been developed for mice and Mongolian gerbils. This technical note proposes a detailed and optimised immunofluorescence protocol in the Mongolian gerbil comprising significant quantitative and qualitative improvements. This protocol sequentially comprises: fixation (1 day), decalcification (6 days), pre-treatment (7.5hours), immunolabelling (42hours), dehydration and clearing (23hours), followed by mounting and laser scanning confocal microscopy acquisition. This protocol has been optimised in terms of duration (10 days versus 13 days) with a reduction of the number of steps, improvement of the specificity of immunolabelling and optimisation of the quality of the results obtained. This technical note provides a detailed description of this protocol.Show less >
Show more >Immunofluorescence on cleared intact cochlea allows detailed analysis of the cochlear ultrastructure, while avoiding the problems of dissection and serial sections. Protocols have been developed for mice and Mongolian gerbils. This technical note proposes a detailed and optimised immunofluorescence protocol in the Mongolian gerbil comprising significant quantitative and qualitative improvements. This protocol sequentially comprises: fixation (1 day), decalcification (6 days), pre-treatment (7.5hours), immunolabelling (42hours), dehydration and clearing (23hours), followed by mounting and laser scanning confocal microscopy acquisition. This protocol has been optimised in terms of duration (10 days versus 13 days) with a reduction of the number of steps, improvement of the specificity of immunolabelling and optimisation of the quality of the results obtained. This technical note provides a detailed description of this protocol.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Collections :
Submission date :
2021-01-20T15:59:21Z