Discovery of a new sialic acid binding ...
Document type :
Article dans une revue scientifique
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Title :
Discovery of a new sialic acid binding region that regulates Siglec-7
Author(s) :
Yamakawa, Nao [Auteur]
Nagoya University
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Yasuda, Yu [Auteur]
Nagoya University
Yoshimura, Atsushi [Auteur]
Nagoya University
Goshima, Ami [Auteur]
Nagoya University
Crocker, Paul R. [Auteur]
University of Dundee
Vergoten, Gerard [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Nishiura, Yuji [Auteur]
Tokyo Institute of Technology [Tokyo] [TITECH]
Takahashi, Takashi [Auteur]
Hanashima, Shinya [Auteur]
Osaka University [Osaka]
Matsumoto, Kana [Auteur]
Yamaguchi, Yoshiki [Auteur]
Tanaka, Hiroshi [Auteur]
Tokyo Institute of Technology [Tokyo] [TITECH]
Kitajima, Ken [Auteur]
Sato, Chihiro [Auteur]
Nagoya University
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Yasuda, Yu [Auteur]
Nagoya University
Yoshimura, Atsushi [Auteur]
Nagoya University
Goshima, Ami [Auteur]
Nagoya University
Crocker, Paul R. [Auteur]
University of Dundee
Vergoten, Gerard [Auteur]
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Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Nishiura, Yuji [Auteur]
Tokyo Institute of Technology [Tokyo] [TITECH]
Takahashi, Takashi [Auteur]
Hanashima, Shinya [Auteur]
Osaka University [Osaka]
Matsumoto, Kana [Auteur]
Yamaguchi, Yoshiki [Auteur]
Tanaka, Hiroshi [Auteur]
Tokyo Institute of Technology [Tokyo] [TITECH]
Kitajima, Ken [Auteur]
Sato, Chihiro [Auteur]
Journal title :
Scientific Reports
Abbreviated title :
Sci Rep
Volume number :
10
Publisher :
Springer Science and Business Media LLC
Publication date :
2020-05-26
ISSN :
2045-2322
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Siglec-7 is a human CD33-like siglec, and is localised predominantly on human natural killer (NK) cells and monocytes. Siglec-7 is considered to function as an immunoreceptor in a sialic acid-dependent manner. However, the ...
Show more >Siglec-7 is a human CD33-like siglec, and is localised predominantly on human natural killer (NK) cells and monocytes. Siglec-7 is considered to function as an immunoreceptor in a sialic acid-dependent manner. However, the underlying mechanisms linking sialic acid-binding and function remain unknown. Here, to gain new insights into the ligand-binding properties of Siglec-7, we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-binding region (site 2 containing R67) in addition to the well-known primary ligand-binding region (site 1 containing R124). This was supported by equilibrium dialysis, STD-NMR experiments, and inhibition analysis of GD3-binding toward Siglec-7 using synthetic sialoglycoconjugates and a comprehensive set of ganglioside-based glycoconjugates. Our results suggest that the two ligand-binding sites are potentially controlled by each other due to the flexible conformation of the C-C′ loop of Siglec-7.Show less >
Show more >Siglec-7 is a human CD33-like siglec, and is localised predominantly on human natural killer (NK) cells and monocytes. Siglec-7 is considered to function as an immunoreceptor in a sialic acid-dependent manner. However, the underlying mechanisms linking sialic acid-binding and function remain unknown. Here, to gain new insights into the ligand-binding properties of Siglec-7, we carried out in silico analysis and site-directed mutagenesis, and found a new sialic acid-binding region (site 2 containing R67) in addition to the well-known primary ligand-binding region (site 1 containing R124). This was supported by equilibrium dialysis, STD-NMR experiments, and inhibition analysis of GD3-binding toward Siglec-7 using synthetic sialoglycoconjugates and a comprehensive set of ganglioside-based glycoconjugates. Our results suggest that the two ligand-binding sites are potentially controlled by each other due to the flexible conformation of the C-C′ loop of Siglec-7.Show less >
Language :
Anglais
Audience :
Non spécifiée
Administrative institution(s) :
Université de Lille
CNRS
CNRS
Research team(s) :
Diversité structurale des héparanes sulfates et régulation de la réponse inflammatoire
Submission date :
2021-03-23T12:48:04Z
2021-03-24T11:16:23Z
2021-03-24T11:16:23Z
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