The expression and functional analysis of ...
Type de document :
Article dans une revue scientifique
URL permanente :
Titre :
The expression and functional analysis of the sialyl-T antigen in prostate cancer
Auteur(s) :
Bai, Ruifeng [Auteur]
Jilin University [JLU]
Luan, Xue [Auteur]
Jilin University [JLU]
Zhang, Yu [Auteur]
Jilin University [JLU]
Masselot, Catherine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Brockhausen, Inka [Auteur]
Queen's University [Kingston, Canada]
Gao, Yin [Auteur]
Queen's University [Kingston, Canada]
Jilin University [JLU]
Jilin University [JLU]
Luan, Xue [Auteur]
Jilin University [JLU]
Zhang, Yu [Auteur]
Jilin University [JLU]
Masselot, Catherine [Auteur]
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Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Brockhausen, Inka [Auteur]
Queen's University [Kingston, Canada]
Gao, Yin [Auteur]
Queen's University [Kingston, Canada]
Jilin University [JLU]
Titre de la revue :
Glycoconjugate Journal
Nom court de la revue :
Glycoconj J
Numéro :
37
Pagination :
423-433
Éditeur :
Springer Science and Business Media LLC
Date de publication :
2020-06-24
Mot(s)-clé(s) en anglais :
Prostate cancer
Glycosylation
Mass spectrometry
α2,3-sialyltransferase
Sialyl-3T antigen
Glycosylation
Mass spectrometry
α2,3-sialyltransferase
Sialyl-3T antigen
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of thecompositions and functions of glycans is critical for discovering diagnostic biomarkers and ...
Lire la suite >Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of thecompositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer. In thisstudy, we used MALDI-TOF-MS analysis to determine theO-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived fromnormal prostatic tissue. Prostate cancer (CaP) cells exhibited an elevation of simple/shortO-glycans, with a reduction of complexO-glycans, increasedO-glycan sialylation and decreased fucosylation. Core 1 sialylation was increased dramatically in all CaPcells, and especially in PC-3 cells. The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product ofα2,3-sialyltransferase-I (ST3Gal-I) was substantially increased. We therefore focused on exploring the possible function ofST3Gal-I in PC-3 cells. ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migrationand apoptosis. Furtherin vivostudies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mousemodel, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be atarget for therapeutic intervention in cancer treatment.Lire moins >
Lire la suite >Aberrant glycosylation is a featured characteristic of cancer and plays a role in cancer pathology; thus an understanding of thecompositions and functions of glycans is critical for discovering diagnostic biomarkers and therapeutic targets for cancer. In thisstudy, we used MALDI-TOF-MS analysis to determine theO-glycan profiles of prostate cancer cells metastasized to bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP) in comparison to immortalized RWPE-1 cells derived fromnormal prostatic tissue. Prostate cancer (CaP) cells exhibited an elevation of simple/shortO-glycans, with a reduction of complexO-glycans, increasedO-glycan sialylation and decreased fucosylation. Core 1 sialylation was increased dramatically in all CaPcells, and especially in PC-3 cells. The expression of Neu5Acα2-3Galβ1-3GalNAc- (sialyl-3T antigen) which is the product ofα2,3-sialyltransferase-I (ST3Gal-I) was substantially increased. We therefore focused on exploring the possible function ofST3Gal-I in PC-3 cells. ST3Gal-I silencing studies showed that ST3Gal-I was associated with PC-3 cell proliferation, migrationand apoptosis. Furtherin vivostudies demonstrated that down regulation of ST3Gal-I reduced the tumor size in xenograft mousemodel, indicating that sialyl-3T can serve as a biomarker for metastatic prostate cancer prognosis, and that ST3Gal-I could be atarget for therapeutic intervention in cancer treatment.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
Université de Lille
CNRS
CNRS
Équipe(s) de recherche :
Génétique des enveloppes bactériennes
Date de dépôt :
2021-03-23T14:06:41Z
2021-03-24T07:10:43Z
2021-03-24T07:10:43Z