Novel role for the Golgi membrane protein ...
Type de document :
Article dans une revue scientifique
URL permanente :
Titre :
Novel role for the Golgi membrane protein TMEM165 in control of migration and invasion for breast carcinoma
Auteur(s) :
Murali, Pavitra [Auteur]
University of Oklahoma Health Sciences Center [OUHSC]
Johnson, Blake P. [Auteur]
Lu, Zhongpeng [Auteur]
University of Oklahoma Health Sciences Center [OUHSC]
Climer, Leslie [Auteur]
University of Arkansas at Little Rock
Scott, Danielle A. [Auteur]
Medical University of South Carolina [Charleston] [MUSC]
Foulquier, Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Oprea-Ilies, Gabriela [Auteur]
Emory University School of Medicine
Lupashin, Vladimir [Auteur]
University of Arkansas at Little Rock
Drake, Richard R. [Auteur]
Medical University of South Carolina [Charleston] [MUSC]
Abbott, Karen L. [Auteur]
University of Oklahoma Health Sciences Center [OUHSC]
University of Oklahoma Health Sciences Center [OUHSC]
Johnson, Blake P. [Auteur]
Lu, Zhongpeng [Auteur]
University of Oklahoma Health Sciences Center [OUHSC]
Climer, Leslie [Auteur]
University of Arkansas at Little Rock
Scott, Danielle A. [Auteur]
Medical University of South Carolina [Charleston] [MUSC]
Foulquier, Francois [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Oprea-Ilies, Gabriela [Auteur]
Emory University School of Medicine
Lupashin, Vladimir [Auteur]
University of Arkansas at Little Rock
Drake, Richard R. [Auteur]
Medical University of South Carolina [Charleston] [MUSC]
Abbott, Karen L. [Auteur]
University of Oklahoma Health Sciences Center [OUHSC]
Titre de la revue :
Oncotarget
Nom court de la revue :
Oncotarget
Numéro :
11
Pagination :
2747-2762
Éditeur :
Impact Journals, LLC
Date de publication :
2020-07-14
ISSN :
1949-2553
Discipline(s) HAL :
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
The TMEM165 gene encodes for a multiple pass membrane protein localized in the Golgi that has been linked to congenital disorders of glycosylation. The TMEM165 protein is a putative ion transporter that regulates H+/Ca++/Mn++ ...
Lire la suite >The TMEM165 gene encodes for a multiple pass membrane protein localized in the Golgi that has been linked to congenital disorders of glycosylation. The TMEM165 protein is a putative ion transporter that regulates H+/Ca++/Mn++ homeostasis and pH in the Golgi. Previously, we identified TMEM165 as a potential biomarker for breast carcinoma in a glycoproteomic study using late stage invasive ductal carcinoma tissues with patient- matched adjacent normal tissues. The TMEM165 protein was not detected in non-malignant matched breast tissues and was detected in invasive ductal breast carcinoma tissues by mass spectrometry. Our hypothesis is that the TMEM165 protein confers a growth advantage to breast cancer. In this preliminary study we have investigated the expression of TMEM165 in earlier stage invasive ductal carcinoma and ductal carcinoma in situ cases. We created a CRISPR/Cas9 knockout of TMEM165 in the human invasive breast cancer cell line MDAMB231. Our results indicate that removal of TMEM165 in these cells results in a significant reduction of cell migration, tumor growth, and tumor vascularization in vivo. Furthermore, we find that TMEM165 expression alters the glycosylation of breast cancer cells and these changes promote the invasion and growth of breast cancer by altering the expression levels of key glycoproteins involved in regulation of the epithelial to mesenchymal transition such as E-cadherin. These studies illustrate new potential functions for this Golgi membrane protein in the control of breast cancer growth and invasion.Lire moins >
Lire la suite >The TMEM165 gene encodes for a multiple pass membrane protein localized in the Golgi that has been linked to congenital disorders of glycosylation. The TMEM165 protein is a putative ion transporter that regulates H+/Ca++/Mn++ homeostasis and pH in the Golgi. Previously, we identified TMEM165 as a potential biomarker for breast carcinoma in a glycoproteomic study using late stage invasive ductal carcinoma tissues with patient- matched adjacent normal tissues. The TMEM165 protein was not detected in non-malignant matched breast tissues and was detected in invasive ductal breast carcinoma tissues by mass spectrometry. Our hypothesis is that the TMEM165 protein confers a growth advantage to breast cancer. In this preliminary study we have investigated the expression of TMEM165 in earlier stage invasive ductal carcinoma and ductal carcinoma in situ cases. We created a CRISPR/Cas9 knockout of TMEM165 in the human invasive breast cancer cell line MDAMB231. Our results indicate that removal of TMEM165 in these cells results in a significant reduction of cell migration, tumor growth, and tumor vascularization in vivo. Furthermore, we find that TMEM165 expression alters the glycosylation of breast cancer cells and these changes promote the invasion and growth of breast cancer by altering the expression levels of key glycoproteins involved in regulation of the epithelial to mesenchymal transition such as E-cadherin. These studies illustrate new potential functions for this Golgi membrane protein in the control of breast cancer growth and invasion.Lire moins >
Langue :
Anglais
Audience :
Non spécifiée
Établissement(s) :
Université de Lille
CNRS
CNRS
Équipe(s) de recherche :
Mécanismes moléculaires de la N-glycosylation et pathologies associées
Date de dépôt :
2021-03-23T14:53:58Z
2021-03-24T08:42:44Z
2021-03-24T08:42:44Z
Fichiers
- 27668-1056807-1-PB.pdf
- Version éditeur
- Accès libre
- Accéder au document