Ca 2+ -activated cleavage of ezrin visualised ...
Document type :
Article dans une revue scientifique: Article original
DOI :
PMID :
Title :
Ca 2+ -activated cleavage of ezrin visualised dynamically in living myeloid cells during cell surface area expansion
Author(s) :
Roberts, Rhiannon [Auteur]
Cardiff University
Martin, Marianne [Auteur]
Laboratory of Pathogen Host Interactions [Montpellier] [LPHI]
Marion, Sabrina [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Elumalai, Geetha [Auteur]
Cardiff University
Lewis, Kimberly [Auteur]
Cardiff University
Hallett, Maurice [Auteur correspondant]
Cardiff University
Cardiff University
Martin, Marianne [Auteur]
Laboratory of Pathogen Host Interactions [Montpellier] [LPHI]
Marion, Sabrina [Auteur]
Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 [CIIL]
Elumalai, Geetha [Auteur]
Cardiff University
Lewis, Kimberly [Auteur]
Cardiff University
Hallett, Maurice [Auteur correspondant]
Cardiff University
Journal title :
Journal of Cell Science
Pages :
jcs236968
Publisher :
Company of Biologists
Publication date :
2020-03-06
ISSN :
0021-9533
English keyword(s) :
Ezrin
Phagocytosis
Cell spreading
Myeloid cell
Neutrophil
Ca2+ signalling
Phagocytosis
Cell spreading
Myeloid cell
Neutrophil
Ca2+ signalling
HAL domain(s) :
Sciences du Vivant [q-bio]/Immunologie/Immunité innée
Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC]
Sciences du Vivant [q-bio]/Biologie cellulaire/Organisation et fonctions cellulaires [q-bio.SC]
English abstract : [en]
The intracellular events underlying phagocytosis, a crucial event for innate immunity, are still unresolved. In order to test whether the reservoir of membrane required for the formation of the phagocytic pseudopodia is ...
Show more >The intracellular events underlying phagocytosis, a crucial event for innate immunity, are still unresolved. In order to test whether the reservoir of membrane required for the formation of the phagocytic pseudopodia is maintained by cortical ezrin, and that its cleavage is a key step in releasing this membrane, the cleavage of cortical ezrin was monitored within living phagocytes (the phagocytically competent cell line RAW264.7) through expressing two ezrin constructs with fluorescent protein tags located either inside the FERM or at the actin-binding domains. When ezrin is cleaved in the linker region by the Ca2+-activated protease calpain, separation of the two fluorophores would result. Experimentally induced Ca2+ influx triggered cleavage of peripherally located ezrin, which was temporally associated with cell expansion. Ezrin cleavage was also observed in the phagocytic pseudopodia during phagocytosis. Thus, our data demonstrates that peripheral ezrin is cleaved during Ca2+-influx-induced membrane expansion and locally within the extending pseudopodia during phagocytosis. This is consistent with a role for intact ezrin in maintaining folded membrane on the cell surface, which then becomes available for cell spreading and phagocytosis.Show less >
Show more >The intracellular events underlying phagocytosis, a crucial event for innate immunity, are still unresolved. In order to test whether the reservoir of membrane required for the formation of the phagocytic pseudopodia is maintained by cortical ezrin, and that its cleavage is a key step in releasing this membrane, the cleavage of cortical ezrin was monitored within living phagocytes (the phagocytically competent cell line RAW264.7) through expressing two ezrin constructs with fluorescent protein tags located either inside the FERM or at the actin-binding domains. When ezrin is cleaved in the linker region by the Ca2+-activated protease calpain, separation of the two fluorophores would result. Experimentally induced Ca2+ influx triggered cleavage of peripherally located ezrin, which was temporally associated with cell expansion. Ezrin cleavage was also observed in the phagocytic pseudopodia during phagocytosis. Thus, our data demonstrates that peripheral ezrin is cleaved during Ca2+-influx-induced membrane expansion and locally within the extending pseudopodia during phagocytosis. This is consistent with a role for intact ezrin in maintaining folded membrane on the cell surface, which then becomes available for cell spreading and phagocytosis.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Source :
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