Genotoxicity of tungsten carbide–cobalt ...
Type de document :
Article dans une revue scientifique
URL permanente :
Titre :
Genotoxicity of tungsten carbide–cobalt (WC–Co) nanoparticles in vitro: Mechanisms-of-action studies
Auteur(s) :
Moche, Hélène [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Chevalier, Dany [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Vezin, Herve [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Claude, Nancy [Auteur]
Lorge, Elisabeth [Auteur]
Nesslany, Fabrice [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Institut Pasteur de Lille
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Chevalier, Dany [Auteur]
Impact de l'environnement chimique sur la santé humaine - ULR 4483 [IMPECS]
Vezin, Herve [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement (LASIRE) - UMR 8516
Claude, Nancy [Auteur]
Lorge, Elisabeth [Auteur]
Nesslany, Fabrice [Auteur]
IMPact de l'Environnement Chimique sur la Santé humaine (IMPECS) - ULR 4483
Institut Pasteur de Lille
Titre de la revue :
Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Pagination :
15-22
Éditeur :
Elsevier BV
Date de publication :
2015-02
ISSN :
1383-5718
Mot(s)-clé(s) en anglais :
Oxidative DNA damage
Reactive oxygen species
Clastogenic effect
Aneugenic effect
Nanomaterials
Reactive oxygen species
Clastogenic effect
Aneugenic effect
Nanomaterials
Discipline(s) HAL :
Planète et Univers [physics]
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]
Chimie/Chimie théorique et/ou physique
Sciences du Vivant [q-bio]
Résumé en anglais : [en]
We showed previously that tungsten carbide–cobalt (WC–Co) nanoparticles (NP) can be used as a nanoparticulate positive control in some in vitro mammalian genotoxicity assays. Here, we investigate the mechanisms of action ...
Lire la suite >We showed previously that tungsten carbide–cobalt (WC–Co) nanoparticles (NP) can be used as a nanoparticulate positive control in some in vitro mammalian genotoxicity assays. Here, we investigate the mechanisms of action involved in WC–Co NP genotoxicity in L5178Y mouse lymphoma cells and primary human lymphocytes, in vitro. Data from the micronucleus assay coupled with centromere staining and from the chromosome-aberration assay show the involvement of both clastogenic and aneugenic events. Experiments with the formamidopyrimidine DNA glycosylase (FPG)-modified comet assay showed a slight (non-significant) increase in FPG-sensitive sites in the L5178Y mouse lymphoma cells but not in the human lymphocytes. Electron paramagnetic resonance spin-trapping results showed the presence of hydroxyl radicals (radical dotOH) in WC–Co NP suspensions, with or without cells, but with time-dependent production in the presence of cells. However, a significant difference in radical dotOH production was observed between human lymphocytes from two different donors. Using H2O2, we showed that WC–Co NP can participate in Fenton-like reactions. Thus, radical dotOH might be produced either via intrinsic generation by WC–Co NP or through a Fenton-like reaction in the presence of cells.Lire moins >
Lire la suite >We showed previously that tungsten carbide–cobalt (WC–Co) nanoparticles (NP) can be used as a nanoparticulate positive control in some in vitro mammalian genotoxicity assays. Here, we investigate the mechanisms of action involved in WC–Co NP genotoxicity in L5178Y mouse lymphoma cells and primary human lymphocytes, in vitro. Data from the micronucleus assay coupled with centromere staining and from the chromosome-aberration assay show the involvement of both clastogenic and aneugenic events. Experiments with the formamidopyrimidine DNA glycosylase (FPG)-modified comet assay showed a slight (non-significant) increase in FPG-sensitive sites in the L5178Y mouse lymphoma cells but not in the human lymphocytes. Electron paramagnetic resonance spin-trapping results showed the presence of hydroxyl radicals (radical dotOH) in WC–Co NP suspensions, with or without cells, but with time-dependent production in the presence of cells. However, a significant difference in radical dotOH production was observed between human lymphocytes from two different donors. Using H2O2, we showed that WC–Co NP can participate in Fenton-like reactions. Thus, radical dotOH might be produced either via intrinsic generation by WC–Co NP or through a Fenton-like reaction in the presence of cells.Lire moins >
Langue :
Anglais
Comité de lecture :
Oui
Audience :
Internationale
Vulgarisation :
Non
Établissement(s) :
Université de Lille
CNRS
CNRS
Collections :
Équipe(s) de recherche :
Propriétés magnéto structurales des matériaux (PMSM)
Date de dépôt :
2021-06-17T13:51:25Z
2021-09-02T12:53:45Z
2021-09-10T10:22:18Z
2022-11-18T12:08:40Z
2021-09-02T12:53:45Z
2021-09-10T10:22:18Z
2022-11-18T12:08:40Z
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- Moche et al.pdf
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