Title :

H89 Treatment Reduces Intestinal Inflammation and Candida albicans Overgrowth in Mice

Author(s) :

Dumortier, Corentin [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Charlet, Rogatien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Bettaieb, Ali [Auteur]
Laboratoire d'Immunologie et d'Immunothérapie des cancers [Dijon] [LIIC]
Jawhara, Samir [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Journal title :

Microorganisms

Abbreviated title :

Microorganisms

Volume number :

8

Pages :

2039

Publisher :

MDPI AG

Publication date :

2020-12-19

ISSN :

2076-2607

English keyword(s) :

H89
Candida albicans
Escherichia coli
Enterococcus faecalis
Lactobacillus johnsonii
microbiota
DSS
colitis
protein kinase A

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Deregulation of the dynamic crosstalk between the gut microbiota, intestinal epithelial cells, and immune cells is critically involved in the development of inflammatory bowel disease and the overgrowth of opportunistic ...
Show more >Deregulation of the dynamic crosstalk between the gut microbiota, intestinal epithelial cells, and immune cells is critically involved in the development of inflammatory bowel disease and the overgrowth of opportunistic pathogens, including the human opportunistic fungus Candida albicans. In the present study, we assessed the effect of N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide (H89), a protein kinase A inhibitor, on the migration of macrophages to C. albicans through dextran sulphate sodium (DSS)-challenged Caco-2 cells. We also investigated the impact of H89 on intestinal inflammation and C. albicans clearance from the gut, and determined the diversity of the gut microbiota in a murine model of DSS-induced colitis. H89 reduced the migration of macrophages to C. albicans through DSS-challenged Caco-2 cells. In addition, H89 decreased C. albicans viability and diminished the expression of pro-inflammatory cytokines and innate immune receptors in macrophages and colonic epithelial Caco-2 cells. In mice with DSS-induced colitis, H89 attenuated the clinical and histological scores of inflammation and promoted the elimination of C. albicans from the gut. H89 administration to mice decreased the overgrowth of Escherichia coli and Enterococcus faecalis populations while Lactobacillus johnsonii populations increased significantly. Overall, H89 reduced intestinal inflammation and promoted the elimination of C. albicans from the gut.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

ANR Project :

Stimulating Antifungal Innate Immunity

Administrative institution(s) :

Université de Lille
CNRS

Collections :

• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Glycobiology in fungal Pathogenesis and Clinical Applications

Submission date :

2021-07-05T13:59:38Z
2021-07-12T08:29:27Z