Title :

Effective nanoparticle-based nasal vaccine against latent and congenital toxoplasmosis in sheep

Author(s) :

Ducournau, Celine [Auteur]

Moire, Nathalie [Auteur]

Carpentier, Rodolphe [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Cantin, Pauline [Auteur]
Université Lille Nord de France (COMUE)
Herkt, Clement [Auteur]
Université Lille Nord de France (COMUE)
Lantier, Isabelle [Auteur]
Université de Tours [UT]
Betbeder, Didier [Auteur]
Université Lille Nord de France (COMUE)
Dimier-Poisson, Isabelle [Auteur]

Journal title :

Frontiers in Immunology

Abbreviated title :

Front Immunol

Volume number :

11

Pages :

2183

Publication date :

2020-01-01

ISSN :

1664-3224

Keyword(s) :

one-health approach
ovine toxoplasmosis
nasal vaccination
adjuvant free
nanoparticle

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Toxoplasma gondiiis a parasitic protozoan of worldwide distribution, able to infect all warm-blooded animals, but particularly sheep. Primary infection in pregnant sheep leads to millions of abortions and significant ...
Show more >Toxoplasma gondiiis a parasitic protozoan of worldwide distribution, able to infect all warm-blooded animals, but particularly sheep. Primary infection in pregnant sheep leads to millions of abortions and significant economic losses for the livestock industry. Moreover, infected animals constitute the main parasitic reservoir for humans. Therefore, the development of a One-health vaccine seems the best prevention strategy. Following earlier work, a vaccine constituted of total extract ofToxoplasma gondiiproteins (TE) associated with maltodextrin nanoparticles (DGNP) was developed in rodents. In this study we evaluated the ability of this vaccine candidate to protect against latent and congenital toxoplasmosis in sheep. After two immunizations by either intranasal or intradermal route, DGNP/TE vaccine generated specific Th1-cellular immune response, mediated by APC-secretion of IFN-gamma and IL-12. Secretion of IL-10 appeared to regulate this Th1 response for intradermally vaccinated sheep but was absent in intranasally-vaccinated animals. Finally, protection against latent toxoplasmosis and transplacental transmission were explored. Intranasal vaccination led to a marked decrease of brain cysts compared with the non-vaccinated group. This DGNP/TE vaccine administered intranasally conferred a high level of protection against latent toxoplasmosis and its transplacental transmission in sheep, highlighting the potential for development of such a vaccine for studies in other species.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Submission date :

2021-07-06T12:48:04Z
2024-01-26T14:23:30Z