Titre :

Adherent invasive escherichia coli (aiec) strain lf82, but not candida albicans, plays a profibrogenic role in the intestine

Auteur(s) :

Chokr, Dina [Auteur]
Centre de recherche sur l'Inflammation [CRI (UMR_S_1149 / ERL_8252 / U1149)]
Cornu, Marjorie [Auteur]
Lille Inflammation Research International Center (LIRIC) - U995
Neut, Christel [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Bortolus, Clovis [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Charlet, Rogatien [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576
Desreumaux, Pierre [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Speca, Silvia [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Titre de la revue :

Gut pathogens

Nom court de la revue :

Gut Pathog

Numéro :

13

Pagination :

5

Date de publication :

2021-01-28

ISSN :

1757-4749

Mot(s)-clé(s) :

C. albicans
AIEC strain LF82
TGF-beta-stimulated intestinal epithelial cells
Inflammatory bowel disease
Intestinal fibrosis

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

BACKGROUND: Intestinal fibrosis is a frequent complication of Crohn's disease. However, the factors that cause chronicity and promote fibrogenesis are not yet understood. OBJECTIVE: In the present study, we evaluated the ...
Lire la suite >BACKGROUND: Intestinal fibrosis is a frequent complication of Crohn's disease. However, the factors that cause chronicity and promote fibrogenesis are not yet understood. OBJECTIVE: In the present study, we evaluated the profibrotic effects of adherent-invasive Escherichia coli (AIEC) LF82 strain and Candida albicans in the gut. METHODS: Colonic fibrosis was induced in C57BL/6 mice by administration of three cycles of 2.5% (w/v) dextran sulfate sodium (DSS) for 5 weeks. LF82 and C. albicans were administered orally once at the start of each week or each cycle, respectively. Expression of markers of myofibroblast activation was determined in TGF-β1-stimulated human intestinal epithelial cells (IECs). RESULTS: LF82 administration exacerbated fibrosis in DSS-treated mice, revealed by increased colonic collagen deposition and expression of the profibrotic genes Col1a1, Col3a1, Fn1 and Vim. This was accompanied by enhanced gene expression of proinflammatory cytokines and chemokines, as well as more recruited inflammatory cells into the intestine. LF82 also potentiated TGF-β1-stimulated epithelial-mesenchymal transition and myofibroblast activation in IECs, by further inducing gene expression of the main mesenchymal cell markers FN1 and VIM and downregulating the IEC marker OCLN. Proinflammatory cytokines were overexpressed with LF82 in TGF-β1-stimulated IECs. Conversely, C. albicans did not affect intestinal fibrosis progression in DSS-treated mice or myofibroblast activation in TGF-β1-stimulated IECs. CONCLUSIONS: These results demonstrate that AIEC strain LF82, but not C. albicans, may play a major profibrogenic role in the gut.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

CHU Lille
CNRS
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Date de dépôt :

2021-07-06T12:49:07Z
2022-01-20T10:28:20Z