Title :

A pilot clinical study on post-operative recurrence provides biological clues for a role of candida yeasts and fluconazole in crohn's disease

Author(s) :

Sendid, Boualem [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Salvetat, Nicolas [Auteur]
Sys2Diag-Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic [Sys2Diag]
Sarter, Helene [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Loridant, Severine [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Cunisse, Catherine [Auteur]
Service de Biostatistiques [CHRU Lille]
Francois, Nadine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Aijjou, Rachid [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Gelé, Patrick [Auteur]
Service de Biostatistiques [CHRU Lille]
Leroy, Jordan [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Deplanque, Dominique [Auteur]
Jawhara, Samir [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Weissmann, Dinah [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF]
Desreumaux, Pierre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Gower, Corinne [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Colombel, Jean-Frédéric [Auteur]
Service des Maladies de l'Appareil Digestif et de la Nutrition [CHRU Lille]
Poulain, Daniel [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]

Journal title :

Journal of Fungi

Abbreviated title :

J Fungi (Basel)

Volume number :

7

Pages :

324

Publisher :

MDPI

Publication date :

2021-04-22

ISSN :

2309-608X

Keyword(s) :

Candida
immune sensing
s
Crohn&#8217

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Background and aims: This study prompted by growing evidence of the relationship between the yeast Candida albicans and Crohn’s disease (CD) was intended to assess the effect of a 6-month course of the antifungal fluconazole ...
Show more >Background and aims: This study prompted by growing evidence of the relationship between the yeast Candida albicans and Crohn’s disease (CD) was intended to assess the effect of a 6-month course of the antifungal fluconazole (FCZ) on post-operative recurrence of CD. Methods: Mycological samples (mouth swabs and stools) and serum samples were collected from 28 CD patients randomized to receive either FCZ (n = 14) or placebo (n = 14) before surgical resection. Serological analysis focused on levels of calprotectin, anti-glycan antibodies, and antibody markers of C. albicans pathogenic transition. Levels of galectin-3 and mannose binding lectin (MBL) involved in C. albicans sensing and inflammation were also measured. Results: 1, 2, 3, and 6 months after surgery, endoscopy revealed recurrence in 5/12 (41.7%) patients in the FCZ group and 5/9 (55.6%) in the placebo group, the small cohort preventing any clinical conclusions. In both groups, surgery was followed by a marked decrease in C. albicans colonization and biomarkers of C. albicans pathogenic transition decreased to non-significant levels. Anti-glycan antibodies also decreased but remained significant for CD. Galectin-3 and calprotectin also decreased. Conversely, MBL levels, which inversely correlated with anti-C. albicans antibodies before surgery, remained stable. Building biostatistical multivariate models to analyze he changes in antibody and lectin levels revealed a significant relationship between C. albicans and CD. Conclusion: Several combinations of biomarkers of adaptive and innate immunity targeting C. albicans were predictive of CD recurrence after surgery, with area under the curves (AUCs) as high as 0.86. FCZ had a positive effect on biomarkers evolution.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

CHU Lille
CNRS
Inserm
Université de Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Lille Neurosciences & Cognition (LilNCog) - U 1172
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Troubles cognitifs dégénératifs et vasculaires

Submission date :

2021-07-06T12:51:00Z
2023-01-04T09:43:16Z
2023-01-11T09:33:53Z