Title :

Heteroleptic Ruthenium(II) Complexes with Bathophenanthroline and Bathophenanthroline Disulfonate Disodium Salt as Fluorescent Dyes for In-Gel Protein Staining

Author(s) :

Babak, Maria [Auteur correspondant]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Le Faouder, Pauline [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Trivelli, Xavier [Auteur]
Institut Michel Eugène Chevreul - FR 2638 [IMEC]
Venkatesan, Gopalakrishnan [Auteur]
Bezzubov, Stanislav [Auteur]
N. S. Kurnakov Institute of General and Inorganic Chemistry [IGIC]
Kajjout, Mohammed [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Gushchin, Artem [Auteur]
Novosibirsk State Technical University
Nikolaev Institute of Inorganic Chemistry [Novosibirsk] [NIC]
Hanif, Muhammad [Auteur]
Poizat, Olivier [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Vezin, Herve [Auteur]
Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE]
Rolando, Christian [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]

Journal title :

Inorganic Chemistry

Pages :

4527-4535

Publisher :

American Chemical Society

Publication date :

2020-04-06

ISSN :

0020-1669

English keyword(s) :

Polyacrylamide Gels
Sypro-ruby
Electrochemiluminescence
Electrophoresis
Photophysics
Route
Water
Acids

HAL domain(s) :

Chimie

English abstract : [en]

The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent Ru-II tris(bathophenanthroline disulfonate) complex (Ru(BPS)(3)), which is more cost-effective compared to commercial ...
Show more >The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent Ru-II tris(bathophenanthroline disulfonate) complex (Ru(BPS)(3)), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic Ru-II complexes Ru(BPS)(2)(BP) and Ru(BPS)(BP)(2) containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)(3). It was shown by fluorescent and UV-vis measurements that novel Ru-II complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)(2) can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).Show less >

Language :

Anglais

Popular science :

Non

ANR Project :

Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation

Collections :

• Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290

Source :

Harvested from HAL