Differential Expression of Proteins in ...
Document type :
Compte-rendu et recension critique d'ouvrage
DOI :
PMID :
Title :
Differential Expression of Proteins in Response to Ceramide-Mediated Stress Signal in Colon Cancer Cells by 2-D Gel Electrophoresis and MALDI-TOF−MS
Author(s) :
Fillet, M. [Auteur]
Université de Liège
Cren-Olivé, C. [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Renert, A.-F. [Auteur]
Université de Liège
Piette, J. [Auteur]
Institut de Génétique Moléculaire de Montpellier [IGMM]
Vandermoere, Franck [Auteur]
Signalisation des facteurs de croissance dans le cancer du sein. Protéomique fonctionnelle
Rolando, Ch. [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Merville, M-P. [Auteur]
Université de Liège
Université de Liège
Cren-Olivé, C. [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Renert, A.-F. [Auteur]
Université de Liège
Piette, J. [Auteur]
Institut de Génétique Moléculaire de Montpellier [IGMM]
Vandermoere, Franck [Auteur]
Signalisation des facteurs de croissance dans le cancer du sein. Protéomique fonctionnelle
Rolando, Ch. [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]
Merville, M-P. [Auteur]
Université de Liège
Journal title :
Journal of Proteome Research
Pages :
870-880
Publisher :
American Chemical Society
Publication date :
2005-06
ISSN :
1535-3893
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire
Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Génomique, Transcriptomique et Protéomique [q-bio.GN]
English abstract : [en]
Comparative cancer cell proteome analysis is a strategy to study the implication of ceramides in the transmission of stress signals. To better understand the mechanisms by which ceramide regulate some physiological or ...
Show more >Comparative cancer cell proteome analysis is a strategy to study the implication of ceramides in the transmission of stress signals. To better understand the mechanisms by which ceramide regulate some physiological or pathological events and the response to the pharmacological treatment of cancer, we performed a differential analysis of the proteome of HCT-116 (human colon carcinoma) cells in response to these substances. We first established the first 2-dimensional map of the HCT-116 proteome. Then, HCT116 cell proteome treated or not with C6-ceramide have been compared using two-dimensional electrophoresis, matrix-assisted laser desorption/ionization-mass spectrometry and bioinformatic (genomic databases). 2-DE gel analysis revealed more than fourty proteins that were differentially expressed in control cells and cells treated with ceramide. Among them, we confirmed the differential expression of proteins involved in apoptosis and cell adhesion.Show less >
Show more >Comparative cancer cell proteome analysis is a strategy to study the implication of ceramides in the transmission of stress signals. To better understand the mechanisms by which ceramide regulate some physiological or pathological events and the response to the pharmacological treatment of cancer, we performed a differential analysis of the proteome of HCT-116 (human colon carcinoma) cells in response to these substances. We first established the first 2-dimensional map of the HCT-116 proteome. Then, HCT116 cell proteome treated or not with C6-ceramide have been compared using two-dimensional electrophoresis, matrix-assisted laser desorption/ionization-mass spectrometry and bioinformatic (genomic databases). 2-DE gel analysis revealed more than fourty proteins that were differentially expressed in control cells and cells treated with ceramide. Among them, we confirmed the differential expression of proteins involved in apoptosis and cell adhesion.Show less >
Language :
Anglais
Popular science :
Non
Source :
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