Titre :

Optimization of the discrete pulse sequence for two-dimensional FT-ICR mass spectrometry using infrared multiphoton dissociation

Auteur(s) :

Agthoven, Maria A. [Auteur]
Chiron, Lionel [Auteur]
Coutouly, Marie-Aude [Auteur]
Sehgal, Akansha Ashvani [Auteur]
Département de Chimie - ENS-PSL
Université Pierre et Marie Curie - Paris 6 [UPMC]
Pelupessy, Philippe [Auteur]
Université Pierre et Marie Curie - Paris 6 [UPMC]
Delsuc, Marc-André [Auteur]
Institut de Génétique et de Biologie Moléculaire et Cellulaire [IGBMC]
Rolando, Christian [Auteur]
Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 [MSAP]

Titre de la revue :

International Journal of Mass Spectrometry

Pagination :

114-124

Éditeur :

Elsevier

Date de publication :

2014-09

ISSN :

1387-3806

Mot(s)-clé(s) en anglais :

FT-ICR
2-dimensional
IRMPD
FTMS
Pulse sequence
Optimization

Discipline(s) HAL :

Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire

Résumé en anglais : [en]

20 FT-ICR MS, introduced by Handler et al. (Chem. Phys. Lett. 138 (1987) 195), allows one to correlate precursor and fragment ions in complex samples without requiring ion isolation. Recent advances in electronics, computer ...
Lire la suite >20 FT-ICR MS, introduced by Handler et al. (Chem. Phys. Lett. 138 (1987) 195), allows one to correlate precursor and fragment ions in complex samples without requiring ion isolation. Recent advances in electronics, computer capacities, and gas-free in-cell fragmentation techniques open up new perspectives for 2D MICR MS as an analytical technique. The pulse sequence consists of two encoding pulses separated by an incremental delay, followed by an observe pulse. In our previous 20 MICR MS work we used three pulses of equal duration and amplitude. However, signal intensity was low because it was distributed over a series of intense harmonics. Using a simple theoretical model to analytically express ion fragmentation and 2D MICR MS ion trajectories, we obtained a nearly pure signal when the maximum radius of the ions during the encoding pulses is within the laser beam. By adjusting the experimental parameters of the encoding pulses according to the calculation on the same cyclotron radius, we strongly decrease the intensity of harmonic peaks. We also discuss the effect of increasing the amplitude of the observe pulse, which affects precursor and fragment ion peaks differently in terms of signal-to-noise ratio. The 2D mass spectra obtained with the optimized pulse sequence show a much higher signal-to-noise ratio, even without using denoising algorithms.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Internationale

Vulgarisation :

Non

Collections :

• Miniaturisation pour la Synthèse, l'Analyse et la Protéomique (MSAP) - USR 3290

Source :

Harvested from HAL