Molecular characterization of Cryptosporidium ...
Document type :
Article dans une revue scientifique: Article original
PMID :
Title :
Molecular characterization of Cryptosporidium isolates from diarrheal dairy calves in France
Author(s) :
Mammeri, Mohamed [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chenafi, Ilham [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Julien, Christine [Auteur]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]
Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Yncréa Hauts-de-France
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Adjou, Karim Tarik [Auteur correspondant]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chevillot, Aurélie [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Chenafi, Ilham [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Thomas, Myriam [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Julien, Christine [Auteur]
Vallée, Isabelle [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Polack, Bruno [Auteur]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Follet, Jerome [Auteur]

Bio-Micro-Electro-Mechanical Systems - IEMN [BIOMEMS - IEMN]
Yncréa Hauts-de-France
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Adjou, Karim Tarik [Auteur correspondant]
Biologie moléculaire et immunologie parasitaires et fongiques [BIPAR]
Journal title :
Veterinary Parasitology : Regional Studies and Reports
Pages :
100323
Publisher :
Elsevier
Publication date :
2019-12
ISSN :
2405-9390
English keyword(s) :
Cryptosporidium parvum
PCR-RFLP
185 rRNA gene
gp60 subtype
France
Calves
Zoonotic parasite
PCR-RFLP
185 rRNA gene
gp60 subtype
France
Calves
Zoonotic parasite
HAL domain(s) :
Sciences du Vivant [q-bio]/Biologie animale/Médecine vétérinaire et santé animale
English abstract : [en]
Cryptosporidium is an obligate intracellular protist parasite infecting a wide range of vertebrate hosts and causes significant intestinal disease in both animals and humans, as some species are zoonotic. Cattle and ...
Show more >Cryptosporidium is an obligate intracellular protist parasite infecting a wide range of vertebrate hosts and causes significant intestinal disease in both animals and humans, as some species are zoonotic. Cattle and especially calves have been identified as one of the most common reservoirs of this protist. However, little is known about the genetics of Cryptosporidium in calves in some regions of France. The aim of this study was to detect and isolate Cryptosporidium spp. in faecal samples from naturally infected pre-weaned calves (<= 45 days-old) in France. A total of 35 diarrhoeic pre-weaned calf faecal samples were collected from 26 dairy cattle farms in six departments (French administrative provinces). Cryptosporidium presence was established by microscopically screening samples for oocystes with an immunofluorescent (DFA) staining method. DFA-positive samples were then analysed by PCR-RFLP and 18S rRNA gene sequencing to determine species. Cryptosporidium parvum-positive samples were subtyped via nested PCR analysis of a partial fragment of the 60 kDa glycoprotein (gp60) gene product. Data were then integrated into phylogenetic tree analysis. DFA revealed the presence of Cryptosporidium oocysts in 31 out of 35 (88%) samples. Combined with 18S rRNA gene analysis results, C. parvum was detected in 30 samples. Subtyping analysis in 27/30 samples (90%) of the C. parvum isolates revealed two zoonotic subtype families, IIa (24/27) and IId (3/27). Four subtypes were recognised within the subtype family Ha, including the hypertransmissible IIaA15G2R1 subtype that is the most frequently reported worldwide (21/ 27), IIaA17G3R1 (1/27), IIaA17G1R1 (1/27), and IIaA19G1R1 (1/27). Two subtypes were recognised within the IId subtype family including IIdA22G1 (2/27) and IIdA27G1 (1/27). These findings illustrate the high occurrence of Cryptosporidium in calves in dairy herds and increase the diversity of molecularly characterised C. parvum isolates with the first description of IIaA17G3R1, IIaA19G1R1, and IId subtypes in France. The presence of zoonotic C. parvum subtype families (Ha, IId) in this study suggests that pre-weaned calves are likely to be a significant reservoir of zoonotic C. parvum, and highlights the importance of animal to human cryptosporidiosis transmission risk. Further molecular studies in calves and small ruminants from other French regions are required to better understand the epidemiology of cryptosporidiosis in France.Show less >
Show more >Cryptosporidium is an obligate intracellular protist parasite infecting a wide range of vertebrate hosts and causes significant intestinal disease in both animals and humans, as some species are zoonotic. Cattle and especially calves have been identified as one of the most common reservoirs of this protist. However, little is known about the genetics of Cryptosporidium in calves in some regions of France. The aim of this study was to detect and isolate Cryptosporidium spp. in faecal samples from naturally infected pre-weaned calves (<= 45 days-old) in France. A total of 35 diarrhoeic pre-weaned calf faecal samples were collected from 26 dairy cattle farms in six departments (French administrative provinces). Cryptosporidium presence was established by microscopically screening samples for oocystes with an immunofluorescent (DFA) staining method. DFA-positive samples were then analysed by PCR-RFLP and 18S rRNA gene sequencing to determine species. Cryptosporidium parvum-positive samples were subtyped via nested PCR analysis of a partial fragment of the 60 kDa glycoprotein (gp60) gene product. Data were then integrated into phylogenetic tree analysis. DFA revealed the presence of Cryptosporidium oocysts in 31 out of 35 (88%) samples. Combined with 18S rRNA gene analysis results, C. parvum was detected in 30 samples. Subtyping analysis in 27/30 samples (90%) of the C. parvum isolates revealed two zoonotic subtype families, IIa (24/27) and IId (3/27). Four subtypes were recognised within the subtype family Ha, including the hypertransmissible IIaA15G2R1 subtype that is the most frequently reported worldwide (21/ 27), IIaA17G3R1 (1/27), IIaA17G1R1 (1/27), and IIaA19G1R1 (1/27). Two subtypes were recognised within the IId subtype family including IIdA22G1 (2/27) and IIdA27G1 (1/27). These findings illustrate the high occurrence of Cryptosporidium in calves in dairy herds and increase the diversity of molecularly characterised C. parvum isolates with the first description of IIaA17G3R1, IIaA19G1R1, and IId subtypes in France. The presence of zoonotic C. parvum subtype families (Ha, IId) in this study suggests that pre-weaned calves are likely to be a significant reservoir of zoonotic C. parvum, and highlights the importance of animal to human cryptosporidiosis transmission risk. Further molecular studies in calves and small ruminants from other French regions are required to better understand the epidemiology of cryptosporidiosis in France.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Audience :
Internationale
Popular science :
Non
Source :
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