Simulated GI digestion of dietary protein: ...
Document type :
Article dans une revue scientifique
PMID :
28460928
Permalink :
Title :
Simulated GI digestion of dietary protein: Release of new bioactive peptides involved in gut hormone secretion
Author(s) :
Caron, Juliette [Auteur]
Cudennec, Benoit [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Domenger, Dorothee [Auteur]
Belguesmia, Yanath [Auteur]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Flahaut, Christophe [Auteur]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Kouach, Mostafa [Auteur]
LESAGE, JEAN [Auteur]
Environnement Périnatal et Santé - EA 4489
Goossens, Jean Francois [Auteur]
Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Dhulster, Pascal [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Ravallec, Rozenn [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Cudennec, Benoit [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Domenger, Dorothee [Auteur]
Belguesmia, Yanath [Auteur]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Flahaut, Christophe [Auteur]
Institut Charles Viollette (ICV) - EA 7394 [ICV]
Kouach, Mostafa [Auteur]
LESAGE, JEAN [Auteur]
Environnement Périnatal et Santé - EA 4489
Goossens, Jean Francois [Auteur]
Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Dhulster, Pascal [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Ravallec, Rozenn [Auteur]
Institut Charles Viollette (ICV) - ULR 7394
Journal title :
Food research international (Ottawa, Ont.)
Abbreviated title :
Food Res. Int.
Volume number :
89
Pages :
382-390
Publication date :
2016
HAL domain(s) :
Sciences du Vivant [q-bio]
French abstract :
Dietary proteins have been reported to induce a strong feeling of satiety that has been partially explained by gut hormone level increase. Up to date, various protein hydrolysates have demonstrated in vitro and in vivo ...
Show more >Dietary proteins have been reported to induce a strong feeling of satiety that has been partially explained by gut hormone level increase. Up to date, various protein hydrolysates have demonstrated in vitro and in vivo their potential to stimulate gut hormone secretion related to food intake decrease and their mechanisms of action have just started to be resolved. In this context, this study aimed at identifying new peptide sequences involved in gut hormone secretion released by protein in vitro gastrointestinal digestion. Targeted gut hormones were Cholecystokinin (CCK) and Glucagon-Like Peptide 1 (GLP-1). The activity of DPP-IV was also considered as it strongly modulates GLP-1 action. In a previous study, simulated digestion of dietary protein has generated hydrolysates with enhancing effect on CCK and GLP-1 secretion in STC-1 cells as well as DPP-IV inhibitory properties. Successive purification steps were performed to isolate peptide fractions involved in these bioactivities whose sequence was determined by LC-MS-MS. Three peptide sequences ANVST, TKAVEH and KAAVT were pointed out for their stimulating effects on GLP-1 secretion. The sequence VAAA was isolated for its DPP-IV inhibitory properties. Two peptide groups were strongly involved in CCK release sharing a certain occurrence of aromatic amino acid residues.Show less >
Show more >Dietary proteins have been reported to induce a strong feeling of satiety that has been partially explained by gut hormone level increase. Up to date, various protein hydrolysates have demonstrated in vitro and in vivo their potential to stimulate gut hormone secretion related to food intake decrease and their mechanisms of action have just started to be resolved. In this context, this study aimed at identifying new peptide sequences involved in gut hormone secretion released by protein in vitro gastrointestinal digestion. Targeted gut hormones were Cholecystokinin (CCK) and Glucagon-Like Peptide 1 (GLP-1). The activity of DPP-IV was also considered as it strongly modulates GLP-1 action. In a previous study, simulated digestion of dietary protein has generated hydrolysates with enhancing effect on CCK and GLP-1 secretion in STC-1 cells as well as DPP-IV inhibitory properties. Successive purification steps were performed to isolate peptide fractions involved in these bioactivities whose sequence was determined by LC-MS-MS. Three peptide sequences ANVST, TKAVEH and KAAVT were pointed out for their stimulating effects on GLP-1 secretion. The sequence VAAA was isolated for its DPP-IV inhibitory properties. Two peptide groups were strongly involved in CCK release sharing a certain occurrence of aromatic amino acid residues.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Université de Lille
CHU Lille
INRA
ISA
Univ. Artois
Univ. Littoral Côte d’Opale
CHU Lille
INRA
ISA
Univ. Artois
Univ. Littoral Côte d’Opale
Collections :
Research team(s) :
Modélisation biopharmaceutique et pharmacocinétique
Submission date :
2019-02-26T17:11:39Z