Titre :

LR12-peptide quantitation in whole blood by RP-HPLC and intrinsic fluorescence detection: Validation and pharmacokinetic study.

Auteur(s) :

Parent, M [Auteur]
Cibles thérapeutiques, formulation et expertise pré-clinique du médicament [CITHEFOR]
Boudier, A [Auteur]
Cibles thérapeutiques, formulation et expertise pré-clinique du médicament [CITHEFOR]
Maincent, P [Auteur]
Cibles thérapeutiques, formulation et expertise pré-clinique du médicament [CITHEFOR]
Gibot, S [Auteur]
Défaillance Cardiovasculaire Aiguë et Chronique [DCAC]
Ait-Oufella, H [Auteur]
Paris-Centre de Recherche Cardiovasculaire [PARCC - UMR-S U970]
Boufenzer, A [Auteur]
Jolly, L [Auteur]
Défaillance Cardiovasculaire Aiguë et Chronique [DCAC]
Derive, M [Auteur]
Kouach, Mostafa [Auteur]
Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365
Goossens, Jean-Francois [Auteur]
Groupe de Recherche sur les formes Injectables et les Technologies Associées - ULR 7365 [GRITA]
Leroy, P [Auteur]
Cibles thérapeutiques, formulation et expertise pré-clinique du médicament [CITHEFOR]
Clarot, I [Auteur]
Cibles thérapeutiques, formulation et expertise pré-clinique du médicament [CITHEFOR]

Titre de la revue :

Biomedical Chromatography

Nom court de la revue :

Biomed. Chromatogr.

Numéro :

31

Pagination :

e3877

Date de publication :

2017

ISSN :

0269-3879

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

A simple, sensitive, selective and robust HPLC method based on intrinsic fluorescence detection was developed for the quantitation of a dodecapeptide (designated as LR12), inhibitor of Triggering Receptor Expressed on ...
Lire la suite >A simple, sensitive, selective and robust HPLC method based on intrinsic fluorescence detection was developed for the quantitation of a dodecapeptide (designated as LR12), inhibitor of Triggering Receptor Expressed on Myeloid cells-1, in rat whole blood. Sample treatment was optimized using protein precipitation and solid-phase extraction. Chromatographic separation was carried out in a gradient mode using a core-shell C18 column (150 × 4.6 mm, 3.6 μm) with mobile phases of acetonitrile and water containing trifluoroacetic acid at 1.0 mL/min. The method was validated using methodology described by the US Food and Drug Administration guidelines for bioanalytical methods. Linearity was demonstrated within the 50-500 ng/mL range and the lower limit of quantitation was 50 ng/mL. Finally, a preliminary pharmacokinetic study after intraperitoneal injection of LR12 in rats was conducted to evaluate both LR12 monomer and its corresponding disulfide dimer, the main product of degradation. Beyond the fact that this paper describes the first fully validated method for LR12 analysis in blood samples, the approach followed here to optimize pre-analytical steps could be beneficial to develop HPLC and/or MS methods for other pharmaceutical peptides.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Université de Lille
CHU Lille

Collections :

• Groupe de Recherche sur les formes Injectables et les Technologies Associées (GRITA) - ULR 7365

Équipe(s) de recherche :

Modélisation biopharmaceutique et pharmacocinétique

Date de dépôt :

2019-02-26T17:11:42Z
2022-04-13T11:01:21Z