Comparative Study on the Impact of Growth ...
Document type :
Article dans une revue scientifique
PMID :
Title :
Comparative Study on the Impact of Growth Conditions on the Physiology and the Virulence of Pseudomonas aeruginosa Biofilm and Planktonic Cells
Author(s) :
Khelissa, Simon Oussama [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Abdallah, Marwan [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Jama, charafeddine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Barras, Alexandre [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Chihib, Nour-Eddine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Abdallah, Marwan [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Jama, charafeddine [Auteur]
Unité Matériaux et Transformations - UMR 8207 [UMET]
Barras, Alexandre [Auteur]
NanoBioInterfaces - IEMN [NBI - IEMN]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Chihib, Nour-Eddine [Auteur]

Unité Matériaux et Transformations - UMR 8207 [UMET]
Journal title :
Journal of food protection
Pages :
1357-1363
Publisher :
International Association for Food Protection
Publication date :
2019-08-01
ISSN :
0362-028X
English keyword(s) :
Biofilm cells
Microbial risk
Planktonic cells
Pseudomonas aeruginosa
Virulence factors
Microbial risk
Planktonic cells
Pseudomonas aeruginosa
Virulence factors
HAL domain(s) :
Sciences du Vivant [q-bio]
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie
English abstract : [en]
The aim of the present work was to study and compare the effect of growth temperature (20, 30, and 37 degrees C) and surface type (stainless steel and polycarbonate) on the production of virulence factors, such as proteases ...
Show more >The aim of the present work was to study and compare the effect of growth temperature (20, 30, and 37 degrees C) and surface type (stainless steel and polycarbonate) on the production of virulence factors, such as proteases and siderophores, and the risk of surface contamination associated with Pseudomonas aeruginosa biofilm and planktonic cells. The increase of growth temperature from 20 to 37 degrees C increased (approximately twofold) the electronegative charge and the hydrophobicity of the P. aeruginosa biofilm cell surface. P. aeruginosa biofilm cell adhesion to stainless steel and polycarbonate was 5- and 1.5-fold higher than their planktonic counterparts at 20 and 30 degrees C, respectively. The increase of growth temperature from 20 to 37 degrees C increased the production of proteases (twofold) and siderophores (twofold) and the cytotoxicity (up to 30-fold) against the HeLa cell line in the supernatants of P. aeruginosa planktonic and biofilm cultures. This study also highlighted that biofilm and planktonic P. aeruginosa cells exhibited distinct physiological properties with respect to the production of virulence factors and the cytotoxicity against the Hela cell line. Therefore, effective disinfection procedures should be adapted to inactivate bacteria detached from biofilms.Show less >
Show more >The aim of the present work was to study and compare the effect of growth temperature (20, 30, and 37 degrees C) and surface type (stainless steel and polycarbonate) on the production of virulence factors, such as proteases and siderophores, and the risk of surface contamination associated with Pseudomonas aeruginosa biofilm and planktonic cells. The increase of growth temperature from 20 to 37 degrees C increased (approximately twofold) the electronegative charge and the hydrophobicity of the P. aeruginosa biofilm cell surface. P. aeruginosa biofilm cell adhesion to stainless steel and polycarbonate was 5- and 1.5-fold higher than their planktonic counterparts at 20 and 30 degrees C, respectively. The increase of growth temperature from 20 to 37 degrees C increased the production of proteases (twofold) and siderophores (twofold) and the cytotoxicity (up to 30-fold) against the HeLa cell line in the supernatants of P. aeruginosa planktonic and biofilm cultures. This study also highlighted that biofilm and planktonic P. aeruginosa cells exhibited distinct physiological properties with respect to the production of virulence factors and the cytotoxicity against the Hela cell line. Therefore, effective disinfection procedures should be adapted to inactivate bacteria detached from biofilms.Show less >
Language :
Anglais
Peer reviewed article :
Oui
Popular science :
Non
Source :
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