Title :

A single-tube, EuroClonality-inspired, TRG clonality multiplex PCR aids management of patients with enteropathic diseases, including from formaldehyde-fixed, paraffin-embedded tissues.

Author(s) :

Derrieux, Coralie [Auteur]
Trinquand, Amelie [Auteur]
Bruneau, Julie [Auteur]
Verkarre, Virginie [Auteur]
Lhermitte, Ludovic [Auteur]
Alcantara, Marion [Auteur]
Villarese, Patrick [Auteur]
Meresse, Bertrand [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Sibon, David [Auteur]
Hermine, Olivier [Auteur]
Brousse, Nicole [Auteur]
Molina, Thierry [Auteur]
Cellier, Christophe [Auteur]
Cerf-Bensussan, Nadine [Auteur]
Malamut, Georgia [Auteur]
Macintyre, Elizabeth [Auteur]

Journal title :

The Journal of molecular diagnostics

Abbreviated title :

J Mol Diagn

Publication date :

2018-09-27

ISSN :

1943-7811
1943-7811

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Celiac disease is a chronic inflammation of the small intestine with villous atrophy that can become refractory to a gluten-free diet. Two categories of refractory celiac disease can be distinguished by the phenotype ...
Show more >Celiac disease is a chronic inflammation of the small intestine with villous atrophy that can become refractory to a gluten-free diet. Two categories of refractory celiac disease can be distinguished by the phenotype of intraepithelial lymphocytes and the status of TRG genes. Their distinction is important because 30% to 50% of type II but only 0% to 14% of type I evolve to an aggressive enteropathy-associated T-cell lymphoma and therefore require intensive treatment. Currently, differential diagnosis integrates immunohistochemistry, immunophenotyping, and TRG clonality analyses, but each has limitations. A single-tube multiplex TRG PCR (ECN) was prospectively compared to an in-house two-tube TRG PCR (N2T) in 73 samples, including 67 cryopreserved intestine tissues. Thirteen formalin-fixed, paraffin-embedded (FFPE) samples were also analyzed retrospectively. The ECN PCR had comparable efficiency to detect major clonal rearrangements in highly infiltrated tissues from T-cell lymphoproliferative disorders and type II refractory celiac disease and to detect the persistence of minor clones in type II refractory celiac disease follow-up samples. The ECN PCR abolished the risk of amplification of false-positive weak clonal rearrangements in cryopreserved specimens and allowed improved detection of clonal rearrangements in DNA from FFPE samples. The ECN PCR allows robust assessment of cryopreserved and FFPE digestive tissues at diagnosis and follow-up of enteropathies with villous atrophy, thus guiding therapeutic management.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Inserm
Université de Lille
CHU Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Research team(s) :

Inflammatory digestive disease : pathophysiology and therapeutic targets developement

Submission date :

2019-03-01T14:07:57Z