In vivo imaging of the Muc5b gel-forming mucin

Portal, Celine; Gouyer, Valérie; Magnien, Mylene; Plet, Segolene; gottrand, frederic; Desseyn, Jean-Luc

Type de document :

Article dans une revue scientifique

DOI :

10.1038/srep44591

PMID :

28294161

URL permanente :

http://hdl.handle.net/20.500.12210/4568

Titre :

In vivo imaging of the Muc5b gel-forming mucin

Auteur(s) :

Portal, Celine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Gouyer, Valérie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Magnien, Mylene [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Plet, Segolene [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
gottrand, frederic [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]
Desseyn, Jean-Luc [Auteur]

Lille Inflammation Research International Center - U 995 [LIRIC]

Titre de la revue :

Scientific Reports

Nom court de la revue :

Sci Rep

Numéro :

Date de publication :

2017-03-15

Discipline(s) HAL :

Sciences du Vivant [q-bio]

Résumé en anglais : [en]

Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in goblet cell density and in gel-forming mucin production have emerged as sensitive indicators for ...
Lire la suite >Gel-forming mucins are macromolecules produced by goblet cells and responsible for the mucus gel formation. Changes in goblet cell density and in gel-forming mucin production have emerged as sensitive indicators for mucosal diseases. A Muc5b-GFP tagged reporter mouse was used to assess Muc5b production in mouse tissues by immunofluorescence microscopy and fluorescent activity using stereromicroscopy and probe-based confocal laser endomicroscopy. Muc5b production was followed longitudinally by recording the fluorescent activity in vagina and in embryonic lung explants under stimulation by interleukin 13. We show that the GFP is easily visualized in the mouse adult ear, nose, trachea, gallbladder, and cervix. Live Muc5b is also easily monitored in the nasal cavity, trachea and vagina where its production varies during the estrus cycle with a peak at the proestrus phase and in pregnant mice. Explant culture of reporter mouse embryonic whole lung shows that interleukin 13 stimulates Muc5b production. The transgenic Muc5b-GFP mouse is unique and suitable to study the mechanisms that regulate Muc5b production/secretion and mucous cell differentiation by live imaging and can be applied to test drug efficacy in mucosal disease models.Lire moins >

Langue :

Anglais

Audience :

Internationale

Vulgarisation :

Non

Établissement(s) :

Inserm
Université de Lille
CHU Lille

Collections :

Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Équipe(s) de recherche :

Nutritional modulation of inflammation and infection

Date de dépôt :

2019-03-01T14:10:20Z
2019-11-13T12:27:02Z

Fichiers

Portal_SciRep_2017.pdf
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Numéro de version	Lien	Date de modification
2	20.500.12210/4568*	2019-11-13T12:25:45Z
1	20.500.12210/4568.1	2019-03-01T14:10:20Z

In vivo imaging of the Muc5b gel-forming mucin

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