Titre :

Silicon nanotweezers inside liquid for the real time characterization of DNA degradation under radiotherapy treatment

Auteur(s) :

Perret, Grégoire [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Lacornerie, T. [Auteur]
Kumemura, V. [Auteur]
Lafitte, N. [Auteur]
Guillou, H. [Auteur]
Jalabert, L. [Auteur]
Lartigau, E. [Auteur]
Centre Régional de Lutte contre le Cancer Oscar Lambret [Lille] [UNICANCER/Lille]
Fujii, T. [Auteur]
Cleri, Fabrizio [Auteur]
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN]
Fujita, H. [Auteur]
The University of Tokyo [UTokyo]
Collard, Dominique [Auteur]
Laboratory for Integrated Micro Mechatronics Systems [LIMMS]

Titre de la manifestation scientifique :

European Materials Research Society Spring Meeting, E-MRS Spring 2014, Symposium N - Converging technology for nanobio-applications

Ville :

Lille

Pays :

France

Date de début de la manifestation scientifique :

2014

Résumé en anglais : [en]

Tumor cell killing by γ-ray beams in radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. On the other hand, the mechanical behavior of ...
Lire la suite >Tumor cell killing by γ-ray beams in radiotherapy is currently based on a rather empirical understanding of the basic mechanisms and effectiveness of DNA damage by radiation. On the other hand, the mechanical behavior of DNA, e.g., sequence-sensitivity, elastic vs. plastic response, is well understood. However, manipulations are usually performed by AFM or optical tweezers, instruments that can hardly be placed and operate under radiation beams. The Silicon Nano Tweezers (SNT) is a MEMS device for direct manipulation of biomolecules, an excellent candidate for in-beam operation thanks to its tiny size. The SNT comprise two parallel arms ending with sharp tips, designed to trap molecules. The mobile arm is displaced by an electrostatic actuator. The motion is acquired by a position sensor, thus the mechanical characteristic of the trapped molecules are measured in real time. The SNT could be inserted inside a microfluidic cavity in order to allow indirect DNA damage due to radicals produced by H2O irradiation. The experiments are performed with a Cyberknife and we first checked the noise level generated by the 6 MeV radiation on the bare SNT; then we tested the ability to detect DNA bundle alteration inside microfluidic cavity. SNT coupled with microfluidics permits to study the mechanics of DNA damage under ionizing beams for optimized tumor treatment.Lire moins >

Langue :

Anglais

Comité de lecture :

Oui

Audience :

Non spécifiée

Vulgarisation :

Non

Collections :

• Institut d'Électronique, de Microélectronique et de Nanotechnologie (IEMN) - UMR 8520

Source :

Harvested from HAL