Transgenic Mouse Reporter to Study Muc5b In Vivo.
Document type :
Article dans une revue scientifique
PMID :
Permalink :
Title :
Transgenic Mouse Reporter to Study Muc5b In Vivo.
Author(s) :
Desseyn, Jean-Luc [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Portal, Celine [Auteur]
gottrand, Fréderic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Gouyer, Valérie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center - U 995 [LIRIC]
Portal, Celine [Auteur]
gottrand, Fréderic [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Lille Inflammation Research International Center (LIRIC) - U995
Gouyer, Valérie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Journal title :
Annals of the American Thoracic Society
Abbreviated title :
Ann Am Thorac Soc
Volume number :
15
Pages :
S149-S153
Publisher :
ATS Journal
Publication date :
2018-11-01
ISSN :
2329-6933
Keyword(s) :
Muc5b-green fluorescent protein
green fluorescent protein live imaging
interleukin-13
mucins
mucus
green fluorescent protein live imaging
interleukin-13
mucins
mucus
HAL domain(s) :
Sciences du Vivant [q-bio]
English abstract : [en]
Dysregulation of gel-forming mucins is associated with many airway diseases. Better knowledge of the pathophysiological mechanisms linking mucins and respiratory diseases will advance the understanding of their pathogenesis ...
Show more >Dysregulation of gel-forming mucins is associated with many airway diseases. Better knowledge of the pathophysiological mechanisms linking mucins and respiratory diseases will advance the understanding of their pathogenesis and should provide opportunities to develop new therapeutic compounds for treatment. MUC5B and MUC5AC are the two main gel-forming mucins in the respiratory tract. The organization in domains and the expression profile of mouse Muc5b are very similar to those in humans, which makes the mouse a relevant model for studies of the translational activities of human mucins. To assess the in vivo biological functions of Muc5b, a mouse reporter tagged in frame with the green fluorescent protein marker has been engineered by homologous recombination. The proof of concept that this reporter model may be informative for translational studies was confirmed by the finding that interleukin-13 administration in living mice upregulated Muc5b production.Show less >
Show more >Dysregulation of gel-forming mucins is associated with many airway diseases. Better knowledge of the pathophysiological mechanisms linking mucins and respiratory diseases will advance the understanding of their pathogenesis and should provide opportunities to develop new therapeutic compounds for treatment. MUC5B and MUC5AC are the two main gel-forming mucins in the respiratory tract. The organization in domains and the expression profile of mouse Muc5b are very similar to those in humans, which makes the mouse a relevant model for studies of the translational activities of human mucins. To assess the in vivo biological functions of Muc5b, a mouse reporter tagged in frame with the green fluorescent protein marker has been engineered by homologous recombination. The proof of concept that this reporter model may be informative for translational studies was confirmed by the finding that interleukin-13 administration in living mice upregulated Muc5b production.Show less >
Language :
Anglais
Audience :
Internationale
Popular science :
Non
Administrative institution(s) :
Inserm
Université de Lille
CHU Lille
Université de Lille
CHU Lille
Research team(s) :
Nutritional modulation of inflammation and infection
Submission date :
2019-03-01T14:34:29Z
2022-06-29T07:23:14Z
2022-06-29T07:23:14Z
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