Title :

β-1,2-Mannosyltransferases 1 and 3 Participate in Yeast and Hyphae O- and N-Linked Mannosylation and Alter Candida albicans Fitness During Infection.

Author(s) :

Courjol, Flavie [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Jouault, Thierry [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Mille, Celine [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Hall, Rebecca [Auteur]
University of Aberdeen
Maes, Emmanuel [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Sendid, Boualem [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Mallet, Jean-Maurice [Auteur]
Laboratoire des biomolécules [LBM UMR 7203]
Guerardel, Yann [Auteur]
Unité de Glycobiologie Structurale et Fonctionnelle UMR 8576 [UGSF]
Gow Neil, A R [Auteur]
University of Aberdeen
Poulain, Daniel [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Fradin, Chantal [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

Open forum infectious diseases

Abbreviated title :

Open Forum Infect. Dis.

Volume number :

2

Pages :

ofv116

Publication date :

2015-09-01

English keyword(s) :

beta-1,2-oligomannosides
fungal virulence
mannosyltransferase
O-mannosylation
Candida albicans

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

β-1,2-mannosylation of Candida albicans glycoconjugates has been investigated through the identification of enzymes involved in the addition of β-1,2-oligomannosides (β-Mans) to phosphopeptidomannan and phospholipomannan. ...
Show more >β-1,2-mannosylation of Candida albicans glycoconjugates has been investigated through the identification of enzymes involved in the addition of β-1,2-oligomannosides (β-Mans) to phosphopeptidomannan and phospholipomannan. β-1,2-oligomannosides are supposed to have virulence properties that they confer to these glycoconjugates. In a previous study, we showed that cell wall mannoproteins (CWMPs) harbor β-Mans in their O-mannosides; therefore, we analyzed their biosynthesis and impact on virulence. In this study, we demonstrate that O-mannans are heterogeneous and that α-mannosylated O-mannosides, which are biosynthesized by Mnt1 and Mnt2 α-1,2-mannosyltransferases, can be modified with β-Mans but only at the nonreducing end of α-1,2-mannotriose. β-1,2-mannosylation of this O-mannotriose depends on growth conditions, and it involves 2 β-1,2-mannosyltransferases, Bmt1 and Bmt3. These Bmts are essential for β-1,2-mannosylation of CWMPs and expression of β-Mans on germ tubes. A bmt1Δ mutant and a mutant expressing no β-Mans unexpectedly disseminated more in BALB/c mice, whereas they had neither attenuated nor enhanced virulence in C57BL/6 mice. In galectin (Gal)3 knockout mice, the reference strain was more virulent than in C57BL/6 mice, suggesting that the β-Mans innate receptor Gal3 is involved in C. albicans fitness during infection.Show less >

Language :

Anglais

Audience :

Internationale

Popular science :

Non

European Project :

Fungi in the setting of inflammation, allergy and autoimmune diseases:Translating basic science into clinical practices

ANR Project :

Structure et fonctions des Beta-1,2 mannosyl transferases de Candida albicans

Administrative institution(s) :

Inserm
Université de Lille
CHU Lille
CNRS

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
• Unité de Glycobiologie Structurale et Fonctionnelle (UGSF) - UMR 8576

Research team(s) :

Fungal associated invasive and inflammatory diseases
Nutritional modulation of inflammation and infection
IBD and environnemental factors : epidemiology and functional analyses

Submission date :

2019-03-01T14:35:02Z
2021-03-25T08:00:41Z