Title :

Toxicological consequences of experimental exposure to aluminum in human intestinal epithelial cells

Author(s) :

Djouina, Madjid [Auteur]
Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286
Lille Inflammation Research International Center - U 995 [LIRIC]
Esquerre, N [Auteur]
Desreumaux, Pierre [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Vignal, Cecile [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]
Body-Malapel, Mathilde [Auteur]
Lille Inflammation Research International Center - U 995 [LIRIC]

Journal title :

Food and Chemical Toxicology

Abbreviated title :

Food Chem. Toxicol.

Volume number :

91

Pages :

108-116

Publication date :

2016-05-01

ISSN :

0278-6915

Keyword(s) :

Mesh:HT29 Cells
Mesh:Humans
Mesh:Intestinal Mucosa/drug effects*
Mesh:Transcriptome
Mesh:Membrane Potentials/drug effects
Mesh:Aluminum/toxicity*
HT-29 cells
Cytotoxicity
Transcriptomics
Aluminum

HAL domain(s) :

Sciences du Vivant [q-bio]

English abstract : [en]

Aluminum (Al), a non-essential element, is ubiquitous in industrialized societies. Whereas adult intake is estimated between 3 and 12 mg/day according to dietary aluminum studies conducted in many countries, it is not known ...
Show more >Aluminum (Al), a non-essential element, is ubiquitous in industrialized societies. Whereas adult intake is estimated between 3 and 12 mg/day according to dietary aluminum studies conducted in many countries, it is not known if aluminum may have a toxic effect on intestinal epithelium. The aim of this work was to evaluate the cytotoxicity and RNA expression patterns induced in HT-29 cells by aluminum. Both classical toxicological methods and a global transcriptomic approach were used. Cytotoxicity determined by MTT assay showed a time and dose dependent decrease of cell viability in aluminum treated cells compared to control cells. Cell cycle analysis by flow cytometry revealed that aluminum induced accumulation of cells in phase G0/G1, associated with a decrease in the proportion of cells in S and G2/M phases. Aluminum led to apoptosis as evidenced by nuclear morphology changes and mitochondrial membrane perturbations, and induced reactive oxygen species generation. Transcriptomic pattern argued in favor of pro-tumorigenic and pro-inflammatory effects of aluminum in intestinal epithelial cells. These results highlight several pathways by which aluminum has a disturbing impact on intestinal epithelial cells, supporting that the effects of aluminum on intestine warrants further investigation.Show less >

Language :

Anglais

Peer reviewed article :

Oui

Audience :

Internationale

Popular science :

Non

Administrative institution(s) :

Inserm
Université de Lille
CHU Lille

Collections :

• Institut de Recherche Translationnelle sur l'Inflammation (INFINITE) - U1286

Research team(s) :

Inflammatory digestive disease : pathophysiology and therapeutic targets developement
IBD and environnemental factors : epidemiology and functional analyses

Submission date :

2019-03-01T14:46:37Z
2019-06-04T14:52:03Z
2019-06-06T11:57:33Z
2021-08-25T15:05:23Z
2021-12-09T10:45:49Z